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Fe3O4 Magnetic nanoparticle peroxidase mimetic-based colorimetric assay for the rapid detection of organophosphorus pesticide and nerve agent.
MedLine Citation:
PMID:  23153113     Owner:  NLM     Status:  Publisher    
Rapid and sensitive detection methods are in urgent demand for the screening of the extensively used organophosphorus pesticides and highly toxic nerve agents for their neurotoxicity. In this study, we developed a novel Fe3O4 magnetic nanoparticles (MNPs) peroxidase mimetic-based colorimetric method for the rapid detection of organophosphorus pesticides and nerve agents. The detection assay is composed of MNPs, acetylcholinesterase (AChE) and choline oxidase (CHO). The enzyme AChE and CHO catalyze the formation of H2O2 in the presence of acetylcholine, which then activates MNPs to catalyze the oxidation of colorimetric substrates to produce a color reaction. After the incubation with the organophosphorus neurotoxins, the enzyme AChE activity was inhibited and produced less H2O2, resulting in a decreased catalytic oxidation of colorimetric substrates over MNPs peroxidase mimetics, accompanied by a drop in color intensity. Three organophosphorus compounds were tested on the assay: acephate and methyl-paraoxon as representative organophosphorus pesticides and nerve agent Sarin. The novel assay displayed substantial color change after incubation in organophosphorus neurotoxins in a concentration-dependent manner. And as low as 1 nM Sarin, 10 nM methyl-paraoxon and 5 µM acephate are easily detected on the novel assay. In conclusion, by employing the peroxidase-mimicking activity of MNPs, the developed colorimetric assay has the potential of becoming a screening tool for the rapid and sensitive assessment of the neurotoxicity of an overwhelming number of organophosphate compounds.
Minmin Liang; Kelong Fan; Yong Pan; Hui Jiang; Fei Wang; Donglin Yang; Di Lu; Jing Feng; Jianjun Zhao; Liu Yang; Xiyun Yan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-16
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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