Document Detail


Fatty acids regulate stress resistance and virulence factor production for Listeria monocytogenes.
MedLine Citation:
PMID:  22843841     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acids (FAs) are the major structural component of cellular membranes, which provide a physical and chemical barrier that insulates intracellular reactions from environmental fluctuations. The native composition of membrane FAs establishes the topological and chemical parameters for membrane-associated functions and is therefore modulated diligently by microorganisms especially in response to environmental stresses. However, the consequences of altered FA composition during host-pathogen interactions are poorly understood. The food-borne pathogen Listeria monocytogenes contains mostly saturated branched-chain FAs (BCFAs), which support growth at low pH and low temperature. In this study, we show that anteiso-BCFAs enhance bacterial resistance against phagosomal killing in macrophages. Specifically, BCFAs protect against antimicrobial peptides and peptidoglycan hydrolases, two classes of phagosome antimicrobial defense mechanisms. In addition, the production of the critical virulence factor, listeriolysin O, was compromised by FA modulation, suggesting that FAs play a key role in virulence regulation. In summary, our results emphasize the significance of FA metabolism, not only in bacterial virulence regulation but also in membrane barrier function by providing resistance against host antimicrobial stress.
Authors:
Yvonne Sun; Brian J Wilkinson; Theodore J Standiford; Henry T Akinbi; Mary X D O'Riordan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-27
Journal Detail:
Title:  Journal of bacteriology     Volume:  194     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-11     Completed Date:  2012-11-23     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5274-84     Citation Subset:  IM    
Affiliation:
University of Michigan Medical School, Department of Microbiology and Immunology, Ann Arbor, Michigan, USA. sunyy@umich.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Fatty Acids / chemistry,  metabolism*
Listeria monocytogenes / genetics,  metabolism*,  pathogenicity
Macrophages / microbiology
Mice
Molecular Structure
Mutation
Stress, Physiological / physiology*
Virulence
Virulence Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
AI064540/AI/NIAID NIH HHS; R01 HL097564/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Virulence Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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