| Fatty acids increase paracellular absorption of aluminium across Caco-2 cell monolayers. | |
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MedLine Citation:
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PMID: 19576870 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Passive paracellular absorption, regulated by tight junctions (TJs), is the main route for absorption of poorly absorbed hydrophilic substances. Surface active substances, such as fatty acids, may enhance absorption of these substances by affecting the integrity of TJ and increasing the permeability. It has been suggested that aluminium (Al) absorption occurs mainly by the paracellular route. Herein, we investigated if physiologically relevant exposures of fully differentiated Caco-2 cell monolayers to oleic acid and docosahexaenoic acid (DHA), which are fatty acids common in food, increase absorption of Al and the paracellular marker mannitol. In an Al toxicity test, mannitol and Al absorption through Caco-2 cell monolayers were similarly modulated by Al concentrations between 1 and 30mM, suggesting that absorption of the two compounds occurred via the same pathways. Exposure of Caco-2 cell monolayers to non-toxic concentrations of Al (2mM) and (14)C-mannitol in fatty acid emulsions (15 and 30mM oleic acid, 5 and 10mM DHA) caused a decreased transepithelial electrical resistance (TEER). Concomitantly, fractional absorption of Al and mannitol, expressed as percentage of apical Al and mannitol retrieved at the basolateral side, increased with increasing dose of fatty acids. Transmission electron microscopy was applied to assess the effect of oleic acid on the morphology of TJ. It was shown that oleic acid caused a less structured morphology of TJ in Caco-2 cell monolayers. Taken together our findings indicate that fatty acids common in food increase the paracellular intestinal absorption of Al. These findings may influence future risk assessment of human Al exposure. |
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Authors:
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Bitte Aspenström-Fagerlund; Birgitta Sundström; Jonas Tallkvist; Nils-Gunnar Ilbäck; Anders W Glynn |
Publication Detail:
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Type: Journal Article Date: 2009-07-02 |
Journal Detail:
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Title: Chemico-biological interactions Volume: 181 ISSN: 1872-7786 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-08-24 Completed Date: 2009-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: Ireland |
Other Details:
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Languages: eng Pagination: 272-8 Citation Subset: IM |
Affiliation:
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Toxicology Division, National Food Administration, Uppsala, Sweden. bfas@slv.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aluminum
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pharmacokinetics* Caco-2 Cells Fatty Acids / pharmacology* Humans Intestinal Absorption / drug effects* Membrane Potentials Microscopy, Electron, Transmission |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 7429-90-5/Aluminum |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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