| Fatty acids from degenerating myelin lipids are conserved and reutilized for myelin synthesis during regeneration in peripheral nerve. | |
| | |
MedLine Citation:
|
PMID: 7561873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Following nerve crush, cholesterol from degenerating myelin is conserved and reutilized for new myelin synthesis during nerve regeneration. The possibility that other myelin lipids are salvaged and reutilized has not been investigated previously. We examined the fate of myelin phospholipids and their fatty acyl moieties following nerve crush by electron microscopic autoradiography of myelin lipids prelabeled with [3H]oleate or [2-3H]-glycerol. Both precursors were incorporated predominantly (> 90%) into phospholipids; > 85% of the [3H]-oleate was incorporated as oleate, with the remainder in longer-chain fatty acids. Before nerve crush, both labels were restricted to myelin sheaths. Following nerve crush and subsequent regeneration, over half the label from [3H]oleate, but little from [2-3H]glycerol, remained in nerve. The oleate label was present as fatty acyl moieties in phospholipids and was localized to newly formed myelin sheaths. Among the extracellular soluble lipids within the degenerating nerve, the bulk of the labeled phospholipids floated at the same density as lipoprotein particles. These data indicate that myelin phospholipids are completely hydrolyzed during nerve degeneration, that at least half the resultant free fatty acids are salvaged and reutilized for new myelin synthesis, and that these salvaged fatty acids are transported by a lipoprotein-mediated mechanism similar to that functioning in cholesterol reutilization. |
| | |
Authors:
|
J F Goodrum; J E Weaver; N D Goines; T W Bouldin |
Related Documents
:
|
15646823 - Altered long chain fatty acids composition in duchenne muscular dystrophy erythrocytes. 2817343 - Quantitative determination of phospholipids using the dyes victoria blue r and b. 1086903 - The role of some small peptides in the transfer of amino nitrogen across the wall of va... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of neurochemistry Volume: 65 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1995 Oct |
Date Detail:
|
Created Date: 1995-10-25 Completed Date: 1995-10-25 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1752-9 Citation Subset: IM |
Affiliation:
|
Brain and Development Research Center, University of North Carolina at Chapel Hill 27599, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Autoradiography Fatty Acids / metabolism* Glycerol / metabolism Lipid Metabolism* Male Myelin Sheath / physiology* Nerve Degeneration / physiology Nerve Regeneration / physiology* Oleic Acid Oleic Acids / metabolism Peripheral Nerves / physiology* Rats Rats, Inbred Strains Solubility |
| Grant Support | |
ID/Acronym/Agency:
|
ES-01104/ES/NIEHS NIH HHS; HD-03110/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids; 0/Oleic Acids; 112-80-1/Oleic Acid; 56-81-5/Glycerol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neurotrophic factors attenuate glutamate-induced accumulation of peroxides, elevation of intracellul...
Next Document: Transglutaminase cross-linking of the tau protein.