Document Detail


Fatty acids do not activate UCP2 in pancreatic beta cells: comparison with UCP1.
MedLine Citation:
PMID:  18626658     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
UCP2 is expressed in pancreatic beta cells where its postulated uncoupling activity will modulate glucose-induced changes in ATP/ADP ratio and insulin secretion. The consequences of UCP2 over/underexpression on beta-cell function has mainly been studied in the basal state; however, a UCP has no uncoupling activity unless stimulated by fatty acids and/or reactive oxygen species. Here, UCP2 was overexpressed in INS-1 cells and parameters reflecting mitochondrial coupling measured in the basal state and after stimulation by fatty acids. For comparison, UCP1 was expressed to similar levels and the same parameters measured. Neither UCP1 expression nor UCP2 overexpression modified basal or glucose-stimulated metabolic changes. Upon addition of fatty acids, UCP1-expressing cells displayed the expected mitochondrial uncoupling effect, while UCP2 did not elicit any measurable change in mitochondrial function. Taken together, our data demonstrate that, in pancreatic beta-cells, UCP2 has no uncoupling activity in the basal state or after fatty acid stimulation.
Authors:
Sandrine Galetti; Alexandre Sarre; Hélène Perreten; Nathalie Produit-Zengaffinen; Patrick Muzzin; Françoise Assimacopoulos-Jeannet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-15
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  457     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-22     Completed Date:  2009-05-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  931-40     Citation Subset:  IM    
Affiliation:
Department of Cell Physiology and Metabolism, Medical Faculty, University of Geneva, 1 rue Michel Servet, 1211, Geneva 4, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism
Adenosine Triphosphate / metabolism
Animals
Cell Line
Fatty Acids / metabolism*
Humans
Insulin / metabolism
Insulin-Secreting Cells / cytology,  metabolism*
Ion Channels
Membrane Potential, Mitochondrial / physiology
Mitochondria / metabolism*
Mitochondrial Proteins
Oxygen Consumption
Rats
Uncoupling Agents / metabolism*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Ion Channels; 0/Mitochondrial Proteins; 0/Uncoupling Agents; 0/mitochondrial uncoupling protein; 0/mitochondrial uncoupling protein 2; 11061-68-0/Insulin; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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