Document Detail


Fatty acids alter monolayer integrity, paracellular transport, and iron uptake and transport in Caco-2 cells.
MedLine Citation:
PMID:  14665727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Caco-2 cell line was used as a model to determine if the type of fatty acid, unsaturated versus saturated, differentially altered the uptake and transport of iron in the human intestine and if the changes were the result of alterations in monolayer integrity and paracellular transport. Cells were cultured in either a lower-iron or normal-iron medium and incubated with a bovine serum albumin control, linoleate, oleate, palmatate, or stearate. Oleate, palmatate, and stearate enhanced (p < 0.05) iron uptake in cells grown in lower iron. The fatty acid effect on iron uptake by cells grown in normal iron was not as pronounced. Iron transport was not affected (p > 0.05) by an interaction between the type of medium (iron concentration) and the type of fatty acid. Iron transport was enhanced (p < 0.05) with palmatate and stearate. Various indicators of monolayer integrity and paracellular transport were also affected by the fatty acids, thus impacting iron uptake and transport. These results indicate that oleate, palmatate, and stearic can enhance iron uptake and transport; however, this enhancement may be the result of alterations in the integrity of the intestine.
Authors:
Elizabeth A Droke; Mary Briske-Anderson; Henry C Lukaski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological trace element research     Volume:  95     ISSN:  0163-4984     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-10     Completed Date:  2004-09-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-32     Citation Subset:  IM    
Affiliation:
Nutritional Sciences Department, Oklahoma State University, Stillwater, OK 74078, USA.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Blood
Caco-2 Cells
Culture Media
Enzymes / metabolism
Fatty Acids / metabolism*
Humans
Iron / metabolism*
Proteins / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Enzymes; 0/Fatty Acids; 0/Proteins; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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