Document Detail

Fatty acid uptake in normal human myocardium.
MedLine Citation:
PMID:  1873878     Owner:  NLM     Status:  MEDLINE    
Fatty acid binding protein has been found in rat aortic endothelial cell membrane. It has been identified to be a 40-kDa protein that corresponds to a 40-kDa fatty acid binding protein with high affinity for a variety of long chain fatty acids isolated from rat heart myocytes. It is proposed that this endothelial membrane fatty acid binding protein might mediate the myocardial uptake of fatty acids. For evaluation of this hypothesis in vivo, influx kinetics of tracer-labeled fatty acids was examined in 15 normal subjects by scintigraphic techniques. Variation of the plasma fatty acid concentration and plasma perfusion rate has been achieved by modulation of nutrition state and exercise conditions. The clinical results suggest that the myocardial fatty acid influx rate is saturable by increasing fatty acid plasma concentration as well as by increasing plasma flow. For analysis of these data, functional relations describing fatty acid transport from plasma into myocardial tissue in the presence and absence of an "unstirred layer" were developed. The fitting of these relations to experimental data indicate that the free fatty acid influx into myocardial tissue reveals the criteria of a reaction on a capillary surface in the vicinity of flowing plasma but not of a reaction in extravascular space or in an unstirred layer and that the fatty acid influx into normal myocardium is a saturable process that is characterized by the quantity corresponding to the Michaelis-Menten constant, Km, and the maximal velocity, Vmax, 0.24 +/- 0.024 mumol/g and 0.37 +/- 0.013 mumol/g(g.min), respectively. These data are compatible with a nondiffusional uptake process mediated by the initial interaction of fatty acids with the 40-kDa membrane fatty acid binding protein of cardiac endothelial cells.
K Vyska; W Meyer; W Stremmel; G Notohamiprodjo; K Minami; H J Machulla; U Gleichmann; H Meyer; R Körfer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Circulation research     Volume:  69     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-09-26     Completed Date:  1991-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  857-70     Citation Subset:  IM    
Department of Molecular Biophysics, University of Bochum, FRG.
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MeSH Terms
Cell Membrane / metabolism
Endothelium, Vascular / metabolism
Exercise Test
Fatty Acids / metabolism*
Heart / radionuclide imaging*
Iodobenzenes / diagnostic use
Models, Cardiovascular
Myocardium / metabolism*
Protein Binding
Thallium Radioisotopes / diagnostic use
Reg. No./Substance:
0/Fatty Acids; 0/Iodobenzenes; 0/Thallium Radioisotopes; 80479-93-2/omega-(4-iodophenyl)pentadecanoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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