| Fatty acid transfer from intestinal fatty acid binding protein to membranes: electrostatic and hydrophobic interactions. | |
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MedLine Citation:
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PMID: 15863832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intestinal fatty acid binding protein (IFABP) is thought to participate in the intracellular transport of fatty acids (FAs). Fatty acid transfer from IFABP to phospholipid membranes is proposed to occur during protein-membrane collisional interactions. In this study, we analyzed the participation of electrostatic and hydrophobic interactions in the collisional mechanism of FA transfer from IFABP to membranes. Using a fluorescence resonance energy transfer assay, we examined the rate and mechanism of transfer of anthroyloxy-fatty acid analogs a) from IFABP to phospholipid membranes of different composition; b) from chemically modified IFABPs, in which the acetylation of surface lysine residues eliminated positive surface charges; and c) as a function of ionic strength. The results show clearly that negative charges on the membrane surface and positive charges on the protein surface are important for establishing the "collisional complex", during which fatty acid transfer occurs. In addition, changes in the hydrophobicity of the protein surface, as well as the hydrophobic volume of the acceptor vesicles, also influenced the rate of fatty acid transfer. Thus, ionic interactions between IFABP and membranes appear to play a primary role in the process of fatty acid transfer to membranes, and hydrophobic interactions can also modulate the rates of ligand transfer. |
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Authors:
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Betina Córsico; Gisela R Franchini; Kuo-Tung Hsu; Judith Storch |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. Date: 2005-05-01 |
Journal Detail:
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Title: Journal of lipid research Volume: 46 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-07-20 Completed Date: 2005-11-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 1765-72 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones Bioquímicas de La Plata, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, calles 60 y 120, 1900-La Plata, Argentina. bcorsico@atlas.med.unlp.edu.ar |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Cell Membrane / metabolism* Fatty Acids / metabolism* Fluorescence Resonance Energy Transfer Hydrophobicity Kinetics Osmolar Concentration Rats Static Electricity |
| Grant Support | |
ID/Acronym/Agency:
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DK-38389/DK/NIDDK NIH HHS; TW-01100/TW/FIC NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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