Document Detail


Fatty acid synthesis in pea root plastids is inhibited by the action of long-chain acyl- coenzyme as on metabolite transporters.
MedLine Citation:
PMID:  11457976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The uptake in vitro of glucose (Glc)-6-phosphate (Glc-6-P) into plastids from the roots of 10- to 14-d-old pea (Pisum sativum L. cv Puget) plants was inhibited by oleoyl-coenzyme A (CoA) concentrations in the low micromolar range (1--2 microM). The IC(50) (the concentration of inhibitor that reduces enzyme activity by 50%) for the inhibition of Glc-6-P uptake was approximately 750 nM; inhibition was reversed by recombinant rapeseed (Brassica napus) acyl-CoA binding protein. In the presence of ATP (3 mM) and CoASH (coenzyme A; 0.3 mM), Glc-6-P uptake was inhibited by 60%, due to long-chain acyl-CoA synthesis, presumably from endogenous sources of fatty acids present in the preparations. Addition of oleoyl-CoA (1 microM) decreased carbon flux from Glc-6-P into the synthesis of starch and through the oxidative pentose phosphate (OPP) pathway by up to 73% and 40%, respectively. The incorporation of carbon from Glc-6-P into fatty acids was not detected under any conditions. Oleoyl-CoA inhibited the incorporation of acetate into fatty acids by 67%, a decrease similar to that when ATP was excluded from incubations. The oleoyl-CoA-dependent inhibition of fatty acid synthesis was attributable to a direct inhibition of the adenine nucleotide translocator by oleoyl-CoA, which indirectly reduced fatty acid synthesis by ATP deprivation. The Glc-6-P-dependent stimulation of acetate incorporation into fatty acids was reversed by the addition of oleoyl-CoA.
Authors:
S R Fox; S Rawsthorne; M J Hills
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  126     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-17     Completed Date:  2001-09-13     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1259-65     Citation Subset:  IM    
Affiliation:
The Department of Brassica and Oilseeds Research, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / pharmacology*
Adenosine Triphosphate / metabolism
Biological Transport
Brassica
Energy Metabolism
Fatty Acids / biosynthesis*
Glucose-6-Phosphate / metabolism
Hexoses / metabolism
Peas / drug effects,  metabolism*
Plant Roots / metabolism
Plastics / metabolism*
Starch / metabolism
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Fatty Acids; 0/Hexoses; 0/Plastics; 56-65-5/Adenosine Triphosphate; 56-73-5/Glucose-6-Phosphate; 9005-25-8/Starch
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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