| Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes. | |
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MedLine Citation:
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PMID: 16682516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fatty acid synthase (FAS) regulates the production of fatty acids and plays a role in regulating apoptosis. Hoechst 33342-induced apoptosis in BC3H-1 myocytes was used as a model to explore intracellular changes in FAS protein (Western blot) and FAS mRNA (RT-PCR). Total lipid and individual phospholipid synthesis was inhibited by a lethal dose of Hoechst 33342 (20 microg/ml) while total lipid and phospholipid degradation ([1-14C]-acetate pulse chase method) were not. Hoechst 33342 at 20 microg/ml reduced the concentration of FAS protein, which was followed more than 6 hr later by a reduction in FAS mRNA. In conclusion, the inhibition of fatty acid synthesis induced by 20 microg/ml of Hoechst 33342 is attributed to the degradation of FAS protein by activated caspases rather than by inhibition of FAS enzyme activity or FAS mRNA synthesis. |
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Authors:
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Xinbo Zhang; Frederick L Kiechle |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of clinical and laboratory science Volume: 36 ISSN: 0091-7370 ISO Abbreviation: Ann. Clin. Lab. Sci. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-05-09 Completed Date: 2006-06-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0410247 Medline TA: Ann Clin Lab Sci Country: United States |
Other Details:
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Languages: eng Pagination: 185-93 Citation Subset: IM |
Affiliation:
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Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / physiology* Benzimidazoles / pharmacology* Cell Line Fatty Acid Synthetase Complex / biosynthesis* Lipid Metabolism / drug effects* Mice Muscle Cells / drug effects*, enzymology, physiology Phospholipids / biosynthesis RNA, Messenger / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Benzimidazoles; 0/Phospholipids; 0/RNA, Messenger; 23491-52-3/HOE 33342; EC 6.-/Fatty Acid Synthetase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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