Document Detail

Fatty acid production in genetically modified cyanobacteria.
MedLine Citation:
PMID:  21482809     Owner:  NLM     Status:  MEDLINE    
To avoid costly biomass recovery in photosynthetic microbial biofuel production, we genetically modified cyanobacteria to produce and secrete fatty acids. Starting with introducing an acyl-acyl carrier protein thioesterase gene, we made six successive generations of genetic modifications of cyanobacterium Synechocystis sp. PCC6803 wild type (SD100). The fatty acid secretion yield was increased to 197 ± 14 mg/L of culture in one improved strain at a cell density of 1.0 × 10(9) cells/mL by adding codon-optimized thioesterase genes and weakening polar cell wall layers. Although these strains exhibited damaged cell membranes at low cell densities, they grew more rapidly at high cell densities in late exponential and stationary phase and exhibited less cell damage than cells in wild-type cultures. Our results suggest that fatty acid secreting cyanobacteria are a promising technology for renewable biofuel production.
Xinyao Liu; Jie Sheng; Roy Curtiss
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-11
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-27     Completed Date:  2011-06-24     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6899-904     Citation Subset:  IM    
Center for Infectious Diseases and Vaccinology, and Center for Environmental Biotechnology, the Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
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MeSH Terms
Escherichia coli Proteins / biosynthesis*,  genetics
Fatty Acids / genetics,  secretion*
Lysophospholipase / biosynthesis*,  genetics
Organisms, Genetically Modified / genetics,  metabolism*
Periplasmic Proteins / biosynthesis*,  genetics
Synechocystis / enzymology*,  genetics
Thiolester Hydrolases / biosynthesis*,  genetics
Reg. No./Substance:
0/Biofuels; 0/Escherichia coli Proteins; 0/Fatty Acids; 0/Periplasmic Proteins; EC 3.1.1.-/tesA protein, E coli; EC; EC 3.1.2.-/Thiolester Hydrolases; EC hydrolase

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