| Fatty acid production in genetically modified cyanobacteria. | |
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MedLine Citation:
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PMID: 21482809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To avoid costly biomass recovery in photosynthetic microbial biofuel production, we genetically modified cyanobacteria to produce and secrete fatty acids. Starting with introducing an acyl-acyl carrier protein thioesterase gene, we made six successive generations of genetic modifications of cyanobacterium Synechocystis sp. PCC6803 wild type (SD100). The fatty acid secretion yield was increased to 197 ± 14 mg/L of culture in one improved strain at a cell density of 1.0 × 10(9) cells/mL by adding codon-optimized thioesterase genes and weakening polar cell wall layers. Although these strains exhibited damaged cell membranes at low cell densities, they grew more rapidly at high cell densities in late exponential and stationary phase and exhibited less cell damage than cells in wild-type cultures. Our results suggest that fatty acid secreting cyanobacteria are a promising technology for renewable biofuel production. |
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Authors:
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Xinyao Liu; Jie Sheng; Roy Curtiss |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-04-11 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-27 Completed Date: 2011-06-24 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 6899-904 Citation Subset: IM |
Affiliation:
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Center for Infectious Diseases and Vaccinology, and Center for Environmental Biotechnology, the Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biofuels* Escherichia coli Proteins / biosynthesis*, genetics Fatty Acids / genetics, secretion* Lysophospholipase / biosynthesis*, genetics Organisms, Genetically Modified / genetics, metabolism* Periplasmic Proteins / biosynthesis*, genetics Synechocystis / enzymology*, genetics Thiolester Hydrolases / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Biofuels; 0/Escherichia coli Proteins; 0/Fatty Acids; 0/Periplasmic Proteins; EC 3.1.1.-/tesA protein, E coli; EC 3.1.1.5/Lysophospholipase; EC 3.1.2.-/Thiolester Hydrolases; EC 3.1.2.14/oleoyl-(acyl-carrier-protein) hydrolase |
| Comments/Corrections | |
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