Document Detail

Fatty acid oxidation in bone tissue and bone cells in culture. Characterization and hormonal influences.
MedLine Citation:
PMID:  3325035     Owner:  NLM     Status:  MEDLINE    
Fatty acid oxidation and its hormonal modulation were investigated in cultured rat calvaria and in cultivated cell populations. The latter were obtained from calvaria of newborn rats by sequential time-dependent digestion with collagenase, yielding eight cell populations: the early ones containing mainly fibroblasts, the middle ones being osteoblast-like, and late ones osteoblast-osteocyte-like. In calvaria, fatty acid oxidation was increased by adding 0.1 mM- and 1.0 mM-palmitate to the medium, containing 10% (v/v) fetal-calf serum. No effect was found after parathyrin addition in vitro or when injected in vivo. All cell populations obtained by sequential digestion were found to oxidize palmitate, whereby the osteoblast-like cells showed a lower oxidation rate than the other populations. Both parathyrin and calcitonin had no effect on fatty acid oxidation. 1,25-Dihydroxycholecalciferol at 1-100 nM and 24,25-dihydroxycholecalciferol at 100 nM increased oxidation primarily in the population enriched with osteoblast-like cells. Insulin at 1.6 microM diminished it in the cell populations enriched with osteoblast-like cells and in the late bone-cell fraction. However, glucagon had no effect. The energy provided by fatty acid oxidation in this system is approx. 40-80% of glucose metabolism, suggesting that this event may be of importance in the energy metabolism of bone.
G Adamek; R Felix; H L Guenther; H Fleisch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  248     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1988-03-09     Completed Date:  1988-03-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  129-37     Citation Subset:  IM    
Department of Pathophysiology, University of Berne, Switzerland.
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MeSH Terms
Adenosine Triphosphate / metabolism
Bone and Bones / drug effects,  metabolism*
Calcitriol / pharmacology
Cells, Cultured
Culture Techniques
Fatty Acids / metabolism*
Insulin / pharmacology
Lactates / metabolism
Lactic Acid
Oxygen / metabolism
Palmitic Acid
Palmitic Acids / pharmacology
Parathyroid Hormone / pharmacology
Peptide Fragments / pharmacology
Rats, Inbred Strains
Reg. No./Substance:
0/Fatty Acids; 0/Lactates; 0/Palmitic Acids; 0/Parathyroid Hormone; 0/Peptide Fragments; 11061-68-0/Insulin; 32222-06-3/Calcitriol; 50-21-5/Lactic Acid; 52232-67-4/Teriparatide; 56-65-5/Adenosine Triphosphate; 57-10-3/Palmitic Acid; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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