| Fatty acid modulation of cytokine release from human monocytic cells. | |
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MedLine Citation:
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PMID: 8218354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of individual fatty acids on the release of interleukin-1-like (IL-1-like) cytokine activity was investigated on human monocytic cells, primarily the cell line U937 and also peripheral blood monocytes. IL-1-like bioactivity was estimated by assessing the effect of supernatants from monocytic cells on the proliferation of thymocytes as measured by [3H]thymidine incorporation into the acid insoluble fraction of the thymocytes. A pronounced concentration-dependent increase (in the range 1-100 microM) in the release of IL-1-like activity was observed with arachidonic acid, dihomo-gamma-linolenic acid (DGLA) and eicosapentaenoic acid (EPA) in absence and presence of bacterial endotoxin (LPS). A much less pronounced concentration-dependent increase in the release of IL-1-like activity was observed with oleic acid and linoleic acid had only a small effect at 100 microM. The potencies of each fatty for this effect using the EC50 concentrations were arachidonic acid > EPA > or = DGLA > linoleic acid > oleic acid with palmitic acid having no effect. The IL-1-like activity was confirmed by the attenuation of the monocytic-cell-supernatant-induced increase in thymocyte proliferation by anti-IL-1 beta antiserum. An increase in the release of anti-IL-1 beta-antiserum-precipitable radioactivity from U937 cells prelabelled with [35S]methionine then incubated with fatty acids in the presence of LPS further confirmed that IL-1 release was increased. Arachidonic acid and EPA also increased the release of IL-1-like activity from peripheral blood monocytes demonstrating that normal monocytes can respond in a similar manner and that this effect of the fatty acids is not restricted to the U937 cell line. |
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Authors:
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G Baldie; D Kaimakamis; D Rotondo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1179 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1993-12-10 Completed Date: 1993-12-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 125-33 Citation Subset: IM |
Affiliation:
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Department of Biomedical Sciences, Marischal College, University of Aberdeen, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arachidonic Acid
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pharmacology Cell Line Dose-Response Relationship, Drug Eicosapentaenoic Acid / pharmacology Fatty Acids, Unsaturated / pharmacology* Humans Immune Sera / pharmacology Interleukin-1 / immunology, metabolism* Ketoprofen / pharmacology Lipopolysaccharides / pharmacology Monocytes / drug effects*, metabolism Precipitin Tests Sulfur Radioisotopes Tritium |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Immune Sera; 0/Interleukin-1; 0/Lipopolysaccharides; 0/Sulfur Radioisotopes; 10028-17-8/Tritium; 1553-41-9/Eicosapentaenoic Acid; 22071-15-4/Ketoprofen; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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