Document Detail

Fatty acid metabolism during hypothermic perfusion of the isolated dog kidney.
MedLine Citation:
PMID:  531496     Owner:  NLM     Status:  MEDLINE    
Perfusion of isolated dog kidneys was performed at 8--12 degrees C using an albumin solution containing caprylic acid (about 6 mmol/l) and long-chain fatty acids (about 0.5 mmol/l). During 48 h the amounts of caprylic acid in the perfusate fell by about 3 mmol, whereas long-chain FFA increased by 0.2--0.3 mmol and small amounts of arachidonic acid appeared. [14C]palmitate or [14C]linoleate--when added--decreased by about 10% in the perfusate. The decrease was mainly due to exchange with kidney phospholipid fatty acids. Only about 0.4% was recovered as [14C]CO2. The amounts of total phospholipids in kidney tissue decreased by up to 10% during the perfusion (when 10% kidney weight gain was taken into account), whereas lysophospholipids, cholesterol, triglycerides and free fatty acids remained essentially unchanged. The distribution of fatty acids in the total phospholipid fraction was strikingly altered. The relative amounts of arachidonic acid increased, whereas all other major fatty acids decreased. Synthesis of arachidonic acid by chain elongation of perfusate [14C]linoleic acid could not be demonstrated, and no evidence was found for a significant increase of the total amounts of arachidonic acid. The possibility that arachidonic acid-containing phospholipids in the kidney are preferentially preserved during the hypothermic conditions is discussed.
S Skrede; O Slaattelid
Related Documents :
6849946 - Lipid metabolism in chinese hamster v79-r membranes composed of unusual phospholipid mo...
2848806 - Lipid analysis of the glycoinositol phospholipid membrane anchor of human erythrocyte a...
864326 - Experimentally caused proliferation of lysosomes in cultured bhk cells involving an inc...
6615516 - Effect of ethanol on cholesterol and phospholipid composition of hela cells.
21545316 - Synthesis and characterization of new biopolymeric microcapsules containing dehpa-topo ...
17544056 - Low auxotrophy-complementing amino acid concentrations reduce yeast chronological life ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  39     ISSN:  0036-5513     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-04-23     Completed Date:  1980-04-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  765-71     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Arachidonic Acids / analysis
Cholesterol / analysis
Fatty Acids, Nonesterified / analysis,  metabolism*
Hypothermia, Induced
Kidney / metabolism*
Linoleic Acids / analysis
Lipids / analysis
Organ Preservation
Organ Size
Palmitic Acids / analysis
Perfusion / methods
Phospholipids / analysis
Serum Albumin
Triglycerides / analysis
Reg. No./Substance:
0/Arachidonic Acids; 0/Fatty Acids, Nonesterified; 0/Linoleic Acids; 0/Lipids; 0/Palmitic Acids; 0/Phospholipids; 0/Serum Albumin; 0/Triglycerides; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  In vivo haemodynamic evaluation of the Hall-Kaster central flow prosthetic heart valve.
Next Document:  Gastric flow measured with hydrogen clearance technique.