Document Detail


Fatty acid metabolism during hypothermic perfusion of the isolated dog kidney.
MedLine Citation:
PMID:  531496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Perfusion of isolated dog kidneys was performed at 8--12 degrees C using an albumin solution containing caprylic acid (about 6 mmol/l) and long-chain fatty acids (about 0.5 mmol/l). During 48 h the amounts of caprylic acid in the perfusate fell by about 3 mmol, whereas long-chain FFA increased by 0.2--0.3 mmol and small amounts of arachidonic acid appeared. [14C]palmitate or [14C]linoleate--when added--decreased by about 10% in the perfusate. The decrease was mainly due to exchange with kidney phospholipid fatty acids. Only about 0.4% was recovered as [14C]CO2. The amounts of total phospholipids in kidney tissue decreased by up to 10% during the perfusion (when 10% kidney weight gain was taken into account), whereas lysophospholipids, cholesterol, triglycerides and free fatty acids remained essentially unchanged. The distribution of fatty acids in the total phospholipid fraction was strikingly altered. The relative amounts of arachidonic acid increased, whereas all other major fatty acids decreased. Synthesis of arachidonic acid by chain elongation of perfusate [14C]linoleic acid could not be demonstrated, and no evidence was found for a significant increase of the total amounts of arachidonic acid. The possibility that arachidonic acid-containing phospholipids in the kidney are preferentially preserved during the hypothermic conditions is discussed.
Authors:
S Skrede; O Slaattelid
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  39     ISSN:  0036-5513     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-04-23     Completed Date:  1980-04-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  765-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acids / analysis
Cholesterol / analysis
Dogs
Fatty Acids, Nonesterified / analysis,  metabolism*
Hypothermia, Induced
Kidney / metabolism*
Linoleic Acids / analysis
Lipids / analysis
Organ Preservation
Organ Size
Palmitic Acids / analysis
Perfusion / methods
Phospholipids / analysis
Serum Albumin
Triglycerides / analysis
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Fatty Acids, Nonesterified; 0/Linoleic Acids; 0/Lipids; 0/Palmitic Acids; 0/Phospholipids; 0/Serum Albumin; 0/Triglycerides; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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