| Fatty acid-induced modulation of ouabain responsiveness of rat Na, K-ATPase isoforms. | |
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MedLine Citation:
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PMID: 10051686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Membrane phospholipids represent a potential influence on the enzymatic properties of the Na,K-ATPase. Little is known concerning the effects of the fatty acid environment surrounding the enzyme on the kinetic properties of the Na,K-ATPase. We used the most obvious difference among the alpha isoforms of rat, their affinities for digitalis glycosides, to examine the relationship between the lipid environment and the Na,K-ATPase. Specific membrane environments that differ in their fatty acid composition were produced by drug-induced diabetes, as well as variations in diet. The alpha1 isoforms in various tissues were then characterized by their resistance to ouabain in Na,K-ATPase-enriched membrane microsomal fractions. The Na,K-ATPase activity in nerves and hearts were altered by diabetes and partially restored in nerves after a fish oil diet. Evaluation of enzyme kinetics (dose-response curves for ouabain) in membrane preparations allowed us to correlate the ouabain affinity of alpha1 isoform with fatty acid composition. The affinity of the alpha1 isoform for ouabain was significantly increased with accretions in the total amount of fatty acids of the n-6 series (P < 0.0001). Our observations provide a partial explanation for the observed difference in isoform properties among tissues. Moreover, these results underline the interaction between membrane fatty acids and the glycoside binding site of the Na,K-ATPase alpha1 subunit. |
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Authors:
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A Gerbi; J M Maixent |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Journal of membrane biology Volume: 168 ISSN: 0022-2631 ISO Abbreviation: J. Membr. Biol. Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-04-22 Completed Date: 1999-04-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 19-27 Citation Subset: IM |
Affiliation:
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Laboratoire de Recherche Cardiologique, Faculté de Médecine, 15 Bd Pierre Dramard 13015, Marseille, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / chemistry Diabetes Mellitus, Experimental / enzymology Dietary Fats / pharmacology Docosahexaenoic Acids / pharmacology Eicosapentaenoic Acid / pharmacology Enzyme Inhibitors / pharmacology* Fatty Acids / analysis, pharmacology* Fatty Acids, Omega-6 Fatty Acids, Unsaturated / pharmacology Fish Oils / pharmacology Male Membrane Lipids / analysis, physiology* Nerve Tissue Proteins / antagonists & inhibitors Organ Specificity Ouabain / pharmacology* Plant Oils / pharmacology Protein Isoforms / antagonists & inhibitors* Rats Rats, Sprague-Dawley Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors* Streptozocin |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Enzyme Inhibitors; 0/Fatty Acids; 0/Fatty Acids, Omega-6; 0/Fatty Acids, Unsaturated; 0/Fish Oils; 0/Membrane Lipids; 0/Nerve Tissue Proteins; 0/Plant Oils; 0/Protein Isoforms; 1553-41-9/Eicosapentaenoic Acid; 18883-66-4/Streptozocin; 25167-62-8/Docosahexaenoic Acids; 630-60-4/Ouabain; 8001-25-0/olive oil; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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