| Fatty acid and glucose utilization in isolated, working fetal pig hearts. | |
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MedLine Citation:
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PMID: 6869527 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fatty acid uptake and the effects of long- and short-chain fatty acids on glucose utilization were assessed in isolated perfused fetal pig hearts (0.9 gestation) in which oxygenated nutrient buffer was pumped from both ventricles at controlled pressure (55 mmHg, mean arterial pressure) and heart rate (180 beats/min). When either 1.5 mM palmitate or 1.0 mM octanoate was added to buffer containing 10 mM glucose and insulin (100 microU/ml), glucose uptake, as measured by 3H2O production from D-[2-3H]glucose, was suppressed when compared with glucose uptake in the absence of fatty acid. Increased tissue concentrations of glucose 6-phosphate, fructose 6-phosphate, and citrate in hearts perfused with buffer containing octanoate indicated active beta-oxidation and inhibition of phosphofructokinase activity when compared with hearts perfused with glucose alone. In contrast, hearts perfused with buffer containing palmitate showed no increases in these metabolic intermediates. These results suggest that suppression of glucose uptake in the presence of long-chain fatty acid was not the result of phosphofructokinase inhibition but may result from inhibition of glucose transport by palmitate. Determinations of tissue concentrations of free carnitine and carnitine derivatives indicated that, although palmitate underwent esterification to long-chain acyl carnitine in the fetal heart, it failed to undergo extensive beta-oxidation. |
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Authors:
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J C Werner; V Whitman; R R Fripp; H G Schuler; J Musselman; R L Sham |
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Publication Detail:
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Type: Duplicate Publication; In Vitro; Journal Article |
Journal Detail:
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Title: The American journal of physiology Volume: 245 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1983 Jul |
Date Detail:
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Created Date: 1983-08-11 Completed Date: 1983-08-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E19-23 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caprylates / pharmacology Cardiac Output Fatty Acids / metabolism* Female Glucose / metabolism* Heart / embryology, physiology Myocardium / metabolism* Oxygen Consumption Palmitates / pharmacology Swine |
| Chemical | |
Reg. No./Substance:
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0/Caprylates; 0/Fatty Acids; 0/Palmitates; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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