Document Detail


Fatty acid composition of phospholipids in epithelium and stroma of human benign prostatic hyperplasia.
MedLine Citation:
PMID:  9719023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Although it is well known that prostatic 5alpha-reductase is active only in its membrane-bound form, rather limited information is available concerning the composition of cellular lipids in human BPH. Therefore, in the present study, the phospholipid fatty acid composition and content in epithelium and stroma of human BPH have been investigated for the first time. METHODS: Phospholipids separated on TLC plates were methylated and fatty acid methyl esters were analyzed by capillary gas chromatography. RESULTS: The fatty acid composition of total phospholipids was significantly different between epithelium and stroma. In particular, the percentage of oleic acid was significantly higher in epithelium as compared with stroma, whereas that of arachidonic acid was significantly lower in epithelium than in stroma. In addition, significant differences between epithelium and stroma were found in regard to the fatty acid composition of the main phospholipid subclasses. Another remarkable finding were the age-dependent changes of the fatty acid composition in human BPH. CONCLUSIONS: This study shows that the fatty acid composition of phospholipids is significantly different between epithelium and stroma of human BPH. Furthermore, age-dependent alterations of the fatty acid composition were found. Further studies are needed to determine whether the endogenous hormonal milieu in the prostate modulates the fatty acid composition of the prostatic cells, as well as what impact such modulation could have on the properties of membrane proteins, i.e., enzymes like the 5alpha-reductase and receptors, which are thought to be affected by alterations in membrane fluidity or composition, or both.
Authors:
H Weisser; M Krieg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Prostate     Volume:  36     ISSN:  0270-4137     ISO Abbreviation:  Prostate     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-09-09     Completed Date:  1998-09-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8101368     Medline TA:  Prostate     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  235-43     Citation Subset:  IM    
Affiliation:
Institute of Clinical Chemistry, Transfusion and Laboratory Medicine, University Clinic Bergmannsheil, Bochum, Germany. heike.weisser@ruhr-uni-bochum.de
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MeSH Terms
Descriptor/Qualifier:
Acetyltransferases / metabolism
Aged
Aged, 80 and over
Aging / metabolism
Chromatography, Gas
Epithelium / chemistry,  metabolism
Fatty Acid Desaturases / metabolism
Fatty Acids / analysis*,  chemistry,  metabolism
Humans
Male
Middle Aged
Phospholipids / chemistry*,  classification,  metabolism
Prostatic Hyperplasia / metabolism*,  pathology
Substrate Specificity
Testosterone 5-alpha-Reductase / metabolism
Tissue Distribution
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.3.99.5/Testosterone 5-alpha-Reductase; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases

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