| Fatty acid composition of phosphatidylinositol and phosphatidic acid in stimulated platelets. Persistence of arachidonyl-stearyl structure. | |
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MedLine Citation:
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PMID: 6790536 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fatty acid composition of phosphatidylinositol (PI), phosphatidic acid (PA), and the free fatty acid pool of human platelets was studied as a function of time following thrombin stimulation. Upon addition of thrombin, the total amount of fatty acids in PI decreased sharply, then rose toward basal levels, while that of PA showed an inverse pattern. However, the percentage distribution of fatty acids in stimulated as well as unstimulated PI and those in stimulated PA remained relatively constant: stearic and arachidonic acids accounted for 90 and 80% of the total in PI and PA, respectively. These data suggest that in stimulated human platelets PI and PA are interconverted via the "PI cycle." The time course of changes in PI and PA may suggest that the levels of arachidonyl-stearyl PA are involved in regulation of the resynthesis of arachidonyl-steryl PI. In contrast to PI and PA, the free fatty acid pool showed sharp increases in the five major platelet fatty acids: arachidonate, stearate, palmitate, oleate, as well as linoleate. These data suggest that most of the fatty acids liberated upon platelet stimulation are not derived via PI metabolism but by other mechanisms. |
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Authors:
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M J Broekman; J W Ward; A J Marcus |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 256 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1981 Aug |
Date Detail:
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Created Date: 1981-10-25 Completed Date: 1981-10-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 8271-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arachidonic Acid Arachidonic Acids / blood* Blood Platelets / drug effects, metabolism* Fatty Acids, Nonesterified / blood* Humans Kinetics Phosphatidic Acids / blood* Phosphatidylinositols / blood* Stearic Acids / blood* Thrombin / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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HL 18828 06 SCOR/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Fatty Acids, Nonesterified; 0/Phosphatidic Acids; 0/Phosphatidylinositols; 0/Stearic Acids; 506-32-1/Arachidonic Acid; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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