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Fatty acid composition of lipids in pot marigold (Calendula officinalis L.) seed genotypes.
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PMID:  23327299     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Calendula officinalis L. (pot marigold) is an annual aromatic herb with yellow or golden-orange flowers, native to the Mediterranean climate areas. Their seeds contain significant amounts of oil (around 20%), of which about 60% is calendic acid. For these reasons, in Europe concentrated research efforts have been directed towards the development of pot marigold as an oilseed crop for industrial purposes.
RESULTS: The oil content and fatty acid composition of major lipid fractions in seeds from eleven genotypes of pot marigold (Calendula officinalis L.) were determined. The lipid content of seeds varied between 13.6 and 21.7 g oil/100 g seeds. The calendic and linoleic acids were the two dominant fatty acids in total lipid (51.4 to 57.6% and 28.5 to 31.9%) and triacylglycerol (45.7 to 54.7% and 22.6 to 29.2%) fractions. Polar lipids were also characterised by higher unsaturation ratios (with the PUFAs content between 60.4 and 66.4%), while saturates (consisted mainly of palmitic and very long-chain saturated fatty acids) were found in higher amounts in sterol esters (ranging between 49.3 and 55.7% of total fatty acids).
CONCLUSIONS: All the pot marigold seed oils investigated contain high levels of calendic acid (more than 50% of total fatty acids), making them favorable for industrial use. The compositional differences between the genotypes should be considered when breeding and exploiting the pot marigold seeds for nutraceutical and pharmacological purposes.
Authors:
Francisc V Dulf; Doru Pamfil; Adriana D Baciu; Adela Pintea
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Publication Detail:
Type:  Journal Article     Date:  2013-01-17
Journal Detail:
Title:  Chemistry Central journal     Volume:  7     ISSN:  1752-153X     ISO Abbreviation:  Chem Cent J     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-31     Completed Date:  2013-02-01     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  101314213     Medline TA:  Chem Cent J     Country:  England    
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Languages:  eng     Pagination:  8     Citation Subset:  -    
Affiliation:
University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, Cluj-Napoca, 400372, Romania. adriana11_baciu@yahoo.com.
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Journal ID (nlm-ta): Chem Cent J
Journal ID (iso-abbrev): Chem Cent J
ISSN: 1752-153X
Publisher: BioMed Central
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Copyright ©2013 Dulf et al.; licensee Chemistry Central Ltd.
open-access:
Received Day: 30 Month: 9 Year: 2012
Accepted Day: 14 Month: 1 Year: 2013
collection publication date: Year: 2013
Electronic publication date: Day: 17 Month: 1 Year: 2013
Volume: 7First Page: 8 Last Page: 8
PubMed Id: 23327299
ID: 3560196
Publisher Id: 1752-153X-7-8
DOI: 10.1186/1752-153X-7-8

Fatty acid composition of lipids in pot marigold (Calendula officinalis L.) seed genotypes
Francisc V Dulf1 Email: francisc_dulf@yahoo.com
Doru Pamfil1 Email: dpamfil@usamvcluj.ro
Adriana D Baciu1 Email: adriana11_baciu@yahoo.com
Adela Pintea1 Email: adela_pintea@yahoo.com
1University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, Cluj-Napoca, 400372, Romania

Background

Calendula officinalis L. (pot marigold), a member of the Asteraceae family, is an annual aromatic herb with yellow or golden-orange flowers, native to the Mediterranean climate areas, being also successfully cultivated in temperate regions of the Earth for ornamental and medicinal purposes [1]. The species have been reported to contain a variety of phytochemicals, including carbohydrates, lipids, phenolic compounds, steroids, terpenoids, tocopherols, carotenoids and quinones [2-5] with potential health benefits [1,6-10].

Besides the usual fatty acids, a few plants are capable to biosynthesize some unusual fatty acids, with special chemical structure. Usually these fatty acids accumulate in storage tissues, while in green organs they are absent or present in very small amounts. The presence of unusual fatty acids is genetically determined and they are highly significant indicators of phylogenetic relationships [11,12]. The seeds of pot marigold have a significant oil content (around 20%), of which about 60% is the unusual calendic acid (8 t, 10 t, 12c-18:3) [13-16]. Several studies demonstrated that calendic acid is synthesized in Calendula seeds via desaturation of linoleic acid [17-21]. Due to its special structure – with three conjugated double bonds – calendic acid and Calendula seeds oil exhibit interesting chemical and physiological properties.

The seed oils such of Calendula officinalis L., Momordica charantia L. or Aleurites fordii Hemsl., rich in conjugated linolenic acids (CLNAs) have a high rate of oxidation and are used as raw materials in paints and coatings industry, and have applications in the manufacture of cosmetics and some industrial polymers [19,22-24]. For these reasons, in the last few years, a concentrated research effort in Europe has been directed towards the development of Calendula officinalis L. as an oilseed crop for industrial purposes [25] and for the engineering of transgenic plants containing the metabolic route for the conjugated fatty acids biosynthesis [26,27].

The increasing interest for plants producing conjugated fatty acids is also motivated by the recent findings related to their biological effects. It has been shown that CLNAs have an important body fat-lowering effect [28] and possess anti-carcinogenic properties, exhibiting apoptotic activity against a wide variety of tumor cells, such as the U-937 human leukemic cancer cell line and the colon cancer cells (Caco-2) [24,29,30]. Bhaskar et al. [31] observed that the trans CLNAs exhibited stronger growth inhibition and more DNA fragmentation in human colon cancer cells than corresponding cis CLNA isomers.

To our knowledge, all the studies, excepting two short reports of Ul’chenko et al. [32] and Pintea et al. [33], respectively, conducted on marigold seed oils determined the fatty acid contents by analyzing only the total lipid matrix.

Therefore, the aim of the present investigation was to compare the oil content and fatty acid compositions of total lipids (TLs), triacylglycerols (TAGs), polar lipids (PLs) and sterol esters (SEs) in seeds of eleven pot marigold genotypes from six different locations in Europe, grown in the Transylvanian region (Romania). The information obtained is helpful to identify suitable genotypes for use in breeding programs of Calendula officinalis.


Results and discussion
Oil contents

The oil (total lipids) contents in eleven genotypes of pot marigold (Calendula officinalis L.) (CO) seeds are presented in Figure 1.The values were found to vary between 13.6- 21.7 (g oil/100 g seeds). There were no significant differences (p < 0.05) among genotypes, except for oil contents of samples CO4 and CO6 versus CO9. The highest amounts of oils were found in the CO4 (21.7 g/100 g), CO6 (21.5 g/100 g) and CO11 (21.3 g/100 g), whereas the genotypes CO1 (15.5 g/100 g), CO5 (15.3 g/100 g) and CO9 (13.6 g/100 g), exhibited the lowest contents of the TLs. These values were similar to those reported by Cromack and Smith [25] but much higher than those observed by Ozgul- Yucel (5.9% oil in Turkish Calendula seeds) [34] and Angelini et al. (5.4% oil in Italian CO seed crops from 1994) [35]. The TLs content of the analyzed CO seeds in this study were also comparable with those of some non-conventional vegetable oil sources with unique phytochemical compositions, such as bitter gourd (21%), cherry laurel (18.3%), pomegranate (18.1%), blackthorn (16.5%), linseed dodder (15.5-20.7%), and coriander (12.7-18%) seeds [34,35].

Fatty acid composition

The total lipid fatty acid composition as well as the fatty acid composition of TAGs, PLs and SEs of the analyzed pot marigold seed oils is presented in Tables 1 and 2.

TL fatty acids

Nineteen fatty acids were identified in the studied pot marigold seed oils (Figure 2), including very low amounts of a hydroxy fatty acid, namely 9- hydroxy- trans-10, cis-12 octadecadienic-acid (9-HODE).

As expected, calendic acid [18:3 (8 t, 10 t, 12c) (n-6)] was the predominant polyunsaturated fatty acid (PUFA) in all TL extracts, and its composition varied between 51.47% (in CO8) and 57.63% of total fatty acids (in CO4). The next most abundant fatty acid was linoleic acid [18:2 (n-6)] (28.50 to 31.86%), followed by oleic [18:1 (n-9)] (4.44 to 6.25%) and palmitic acids (16:0) (3.86 to 4.55%). Small and very small (or trace) amounts (<2%) of stearic (18:0), β- calendic [18:3 (8 t, 10 t, 12 t) (n-6)], elaidic [18:1 (9 t) (n-9)], arachidic (20:0), behenic (22:0), gondoic [20:1 (n-9)], α- linolenic [18:3 (n-3)], linoelaidicic [18:2 (9 t,12 t) (n-6)], cis-7 hexadecenoic [16:1 (n-9)], palmitoleic [16:1 (n-7)], lauric (12:0), myristic (14:0), pentadecanoic (15:0), and margaric (17:0) acids were also determined. Similar results for the calendic acid content (over 50%) were reported by Cromack and Smith [25] for two of nine hybrids of pot marigold seeds grown in England, as well as by Cahoon et al. [26]. Ozgul- Yucel concluded that Turkish calendula seed oil is characterized by high concentration of linoleic acid (43.5%) and low content of CLNAs (calendic acid (18.3%) + β- calendic (11.2%)) [34]. Moreover, the calendic acid levels reported here are considerably higher than those reported previously by Suzuki et al. [29] (33.4%) and Angelini et al. [35] (16- 46%- in the Italian pot marigold seed oils, crops from 1993).

The available literature shows that the fatty acid composition of oil seeds varies strongly according to their origin/genotype, and geographical/climatic conditions of the growth areas [25,36]. It was also found that the maturity stage of the seeds is an important factor that influences the accumulation of calendic acid in calendula seeds oil. Pintea et al. [33] showed that during the maturation period of the pot marigold seeds (0–2 weeks after flower drops) the concentration of calendic acid increased sharply and steadily (from 8.62% to 53%), accompanied by a decrease in the amounts of linoleic and oleic acids. These observations are in agreement with the presence of the specific conjugase which is able to convert linoleic acid into calendic acid in Calendula seeds [18,26]. The stereospecific analysis of TAG proved that calendic acid preferentially esterifies the sn-2 position of TAG [26,37].

The analysis of fatty acids classes showed statistically significant differences (p < 0.05) with the exception of PUFAs (Figure 3). The highest value of saturated fatty acid (SFAs) (p < 0.05) was registered in the TLs of Czech genotypes (CO11) (7.34%), whereas CO5, CO7 and CO8 were the richest sources of monounsaturated fatty acids (MUFAs) (Figure 3A). On the other hand, small variations (p < 0.05) were found in CLNAs contents (Figure 3B), with the highest proportions in CO4 (58.54%) and the lowest in CO8 (51.95%), respectively. As shown in Table 2, the levels of the PUFAs/SFAs (saturated fatty acids) ratios were significantly higher (p < 0.05) in TLs (due to the high values of 18:3 and 18:2 fatty acids) than in the lipid fractions (TAGs, PLs and SEs) of each pot marigold genotypes.

TAG fatty acids

The fatty acid profiles of the TAGs were similar to that of the profiles of the TL fractions, due to the dominance of the PUFAs (18:3 and 18:2 (n-6) fatty acids) in their compositions (see Tables 1 and 2) and due to the fact that TAG are major components of the seeds oil.

PL and SE fatty acids

The fatty acid composition of the PLs and SEs was different from that of the TL and TAG fractions in all the pot marigold genotypes analyzed (Tables 1 and 2).

The PL fractions were highly unsaturated, with the linoleic acid content ranging from 55.02% (CO2) to 61.51% (CO10) of total fatty acids. Ul’chenko et al. [32] studied the fatty acid compositions of the lipids from seeds, leaves and flowers of Calendula officinalis L. and reported lower value of linoleic acid (24.5%) in the phospholipids of seeds, than those determined in the present work.

With four exceptions (samples CO1-4), the calendic acid content in the PL fractions was lower than 3% (Table 1). This conjugated fatty acid was found to be below 1% in the phosphatidylcholine (PC) of Calendula officinalis seeds oil [26] or was not detected [32]. The differences between the reported data and our data can be explained by the fact that we have investigated the total polar lipids fraction which includes phospholipids and glycolipids. Transgenic soybean and Arabidopsis seeds engineered to synthesize calendic acid (by cloning of the fatty acid conjugase from Calendula) accumulated moderate level of conjugated fatty acids. Calendic acid was found at comparable levels in PC and TAG fractions (85% in the sn-2 position of PC) proving that complex mechanisms involving both desaturation and transacylation processes are involved in the biosynthesis of rich CLNAs enriched TAG [26]. Same authors showed that accumulation of conjugated fatty acids in PC of transgenic plants (soybean and Arabidopsis) negatively affected the appearance and the germination rate of seeds due to the special chemical and physical properties of CLNAs. In consequence, the selection of valuable genotypes of Calendula which are able to produce large amounts of oil enriched in CLNAs still has an economical importance.

The levels of SFAs in SEs were significantly higher (p < 0.05) than in the corresponding lipid fractions of each genotype (Table 2). The amounts of saturated consisted mainly of palmitic (16:0) acid, very long-chain saturated fatty acids (VLCSFAs) (more than 20 carbon atoms) and stearic (18:0) acid, respectively, and varied between 49.31% (in CO1) and 55.74% (in CO8) of total fatty acids from SEs (Tables 1 and 2). These observations are in agreement with the data reported by Zlatanov [38], Kallio et al. [39] and Yang et al. [40] about the fatty acid composition of the phospholipids and the SE fractions of other non-conventional seed oils.

In plant tissues, the very long-chain fatty acids (≥20 carbon atoms) are precursors for the synthesis of lipids, such as cuticular waxes (on the aerial plant surfaces), suberin (embedded in the cell walls of plant-environment interfaces), triacylglycerols (in seeds), and ceramides (in the cell membranes) [41,42].

The TL, TAG and PL fractions of all analyzed pot marigold genotypes exhibited very low proportions of VLCSFAs (<1.50% of total fatty acids), whereas the SEs showed significantly higher (p < 0.05) amounts of this type of fatty acids (from 20.59% (CO4) to 24.78% (CO11)) (Table 2).

As shown in Table 2, in all extracts of pot marigold seeds, the PUFAs/SFAs ratios were significantly lower (p < 0.05) in SE and PL fractions than in the corresponding TLs or TAGs. A comprehensive study of the Diabetes and Nutrition Study Group of the Spanish Diabetes Association showed that a dietary PUFAs/SFAs ratio > 0.4 can greatly reduce the risk of onset of diabetic complications [43]. Moreover, in some earlier reports, the authors indicate that the values of this ratio comprised between 1.0 and 1.5, are optimal to reduce the risk of cardiovascular diseases [44,45]. Thus, the results of the present study show that the Calendula officinalis oil, whatever the genotype analyzed in this paper, may reduce the risk of cardiovascular diseases because both TLs and TAG presented PUFAs/SFAs ratios values are closed to the recommended PUFA/SFA intake by nutrition scientists.


Conclusions

In the present paper, seeds of eleven genotypes of Calendula officinalis L. originating from six different locations in Europe, cultivated in Romania (Transylvanian) were analyzed with respect to oil yields and fatty acid contents. To the best of our knowledge, data about detailed fatty acid composition of main lipid fractions in pot marigold seeds investigated in this study are not available in literature.

The oil content observed in most of the calendula seed samples studied was noted to range between 18 and 22 g oil/100 g seeds. The oil TAGs were similar in fatty acid composition to the TLs, containing substantial amounts of calendic and linoleic acids, making them excellent dietary sources of PUFAs, especially of CLNAs. The PL fractions were highly unsaturated, due to the dominance of the linoleic acid in their structures. A clear characteristic of the SEs from the pot marigold seed oils analyzed were the significantly high levels of SFAs, with considerable amounts of VLCSFAs.

The compositional differences between the genotypes should be considered when breeding and exploiting the calendula seeds for industrial, nutraceutical or pharmacological purposes.


Materials and methods
Seeds and chemicals

Eleven genotypes of Calendula officinalis L. originating from six different locations in Europe (botanical gardens and institutes) (Table 3) were cultivated on experimental fields of the University of Agricultural Sciences and Veterinary Medicine of Cluj- Napoca (Romania). The crops were established in the first half of May 2011, to a target population of 40 plants m-2. Plot area was prepared before (autumn of 2010) by fertilization with animal manure. Nitrogen- based fertilizers were applied during the vegetation period. The seeds were harvested manually at full maturity (end of September-beginning of October).

All reagents (used for the oil extraction, fractionation and fatty acid methyl esters (FAMEs) preparation) and lipid standards (used for identification of the lipid class) were of chromatographic grade (Sigma–Aldrich (St. Louis, MO, USA)). The thin layer chromatography (TLC) plates (silica gel 60 F254, 20 × 20 cm) were purchased from Merck (Darmstadt, Germany).The FAMEs standard (37 component FAME Mix, SUPELCO, catalog No: 47885-U) were purchased from Supelco (Bellefonte, PA, USA).

Oil extraction and fractionation

The oils were extracted from 5 g of seeds, using a methanol/chloroform extraction procedure, according to Yang et al. [36] and Dulf et al. [46]. The sample was homogenized in 50 mL methanol for 1 min using a homogeniser (MICCRA D-9, Germany), 100 mL chloroform was added, and homogenization was continued for further 2 min. The mixture was filtered under vacuum through a Buchner funnel and the solid residue was resuspended in 150 mL of chloroform: methanol (2:1, v/v) and homogenized for another 3 min. The mixture was filtered again and washed with 150 mL chloroform: methanol (2:1, v/v). The filtrates were combined and cleaned with 0.88% potassium chloride water solution and methanol: water (1:1, v/v) solution. The bottom layer (with the purified lipids) was filtered before the solvent was rotary evaporated. The total lipids recovered were transferred to vials with 4 mL chloroform (stock solution), and stored at −18°C for further analysis.

Neutral and polar lipid fractions were separated by TLC [47]. Lipid aliquots (0.2 ml of stock solution) were applied on the TLC plates and then developed in a mixture of petroleum ether: diethyl ether: acetic acid (85:15:1, v/v/v), sprayed with 2’, 7’-dichlorofluoroscein/methanol (0.1% w/v) and viewed under UV light (254 nm) [48]. The lipid classes were identified using commercial standards and then scraped from the TLC plates. The first band (at the origin of the plates), corresponding to the PLs was eluted from silica layer with methanol: chloroform (1:1, v/v), and the upper two major bands of TAGs and SEs respectively were eluted with chloroform. The samples were filtered, the solvent was removed and the dry residue was subjected to transesterification and gas chromatographic (GC) analysis.

Fatty acid analysis

The total lipid, PL, TAG and SE fractions were derivatized by sodium methoxide catalysis [49]. The FAMEs were determined by gas chromatography–mass spectrometry (GC-MS), using a PerkinElmer Clarus 600 T GC-MS (PerkinElmer, Inc., Shelton, U.S.A.) equipped with a, BPx- 70 capillary column (60 m × 0.25 mm i.d., 0.25 μm film; SGE, Ringwood, Australia). The initial oven temperature was 140°C, increased to 220°C with a rate of 2°C/min and then held at this temperature for 25 min. Flow rate of the carrier gas He and the split ratio were 0.8 ml/min and 1:24, respectively. The injector temperature was 210°C. The positive ion electron impact (EI) mass spectra was recorded at an ionization energy of 70 eV and a trap current of 100 μA with a source temperature of 150°C. The mass scans were performed within the range of m/z: 22–395 at a rate of 0.14 scan/s with an intermediate time of 0.02 s between the scans. The injected volume was 0.5 μl. Identification of FAMEs was achieved by comparing their retention times with those of known standards (37component FAME Mix, SUPELCO # 47885-U) and the resulting mass spectra to those in our database (NIST MS Search 2.0).

Statistics

Three different samples of Calendula seeds for each genotype were assayed. The analytical results reported for the fatty acid compositions, are the average of triplicate measurements of three independent oils (n = 3x3). The assumptions of equality of variances and normal distribution of errors were checked for the tested response variables. Since the assumptions were satisfied, data were subjected to one-way ANOVA (repeated measures ANOVA) and Tukey’s post hoc test. Statistical differences among oil samples were estimated using: “Kruskal-Wallis non-parametric test” followed by “Dunn's Multiple Comparison Test” (Graph Pad Prism Version 4.0, Graph Pad Software Inc., San Diego CA). A probability value of p < 0.05 was considered to be statistical significant.


Abbreviations

CLNAs: Conjugated linolenic acids; TLs: Total lipids; TAGs: Triacylglycerols; PLs: Polar lipids; SEs: Sterol esters; PUFAs: Polyunsaturated fatty acids; SFAs: Saturated fatty acids; MUFAs: Monounsaturated fatty acids; VLCSFAs: Very long-chain saturated fatty acids; PC: Phosphatidylcholine; CO: Calendula officinalis; FAMEs: Fatty acid methyl esters; TLC: Thin layer chromatography; GC-MS: Gas chromatography–mass spectrometry.


Competing interests

The authors declare that they have no competing interests.


Authors’ contributions

FVD and DP carried out the experimental design, interpretation of results and preparation of the paper. ADB contributed to the extraction of lipids. AP contributed to the separation, identification and quantification of the lipid fractions and fatty acids from the samples. All authors read and approved the final manuscript.


Acknowledgements

This work was supported by the POSDRU/89/1.5/S/52432 project („Organising the National Interest Postdoctoral School of Applied Biotechnologies with Impact on Romanian Bioeconomy”) and by a grant of the Romanian National Authority for Scientific Research, CNCS – UEFISCDI, project number PN-II-ID-PCE-2011-3-0721.


References
Ercetin T,Senol FS,Orhan IE,Toker G,Comparative assessment of antioxidant and cholinesterase inhibitory properties of the marigold extracts from Calendula arvensis L. and Calendula officinalis LInd Crop ProdYear: 20123620320810.1016/j.indcrop.2011.09.007
Wojciak-Kosior M,Matysik G,Soczewinski E,Investigations of phenolic acids occurring in plant components of Naran N by HPLC and HPTLC densitometric methodsHerba PolYear: 200349194201
Kishimoto S,Maoka T,Sumitomo K,Ohmiya A,Analysis of carotenoid composition in petals of Calendula (Calendula officinalis L.)Biosci Biotechnol BiochemYear: 2005692122212810.1271/bbb.69.212216306694
Re TA,Mooney D,Antignac E,Dufour E,Bark I,Srinivasan V,Nohynek G,Application of the threshold of toxicological concern approach for the safety evaluation of calendula flower (Calendula officinalis) petals and extracts used in cosmetic and personal care productsFood Chem ToxicolYear: 2009471246125410.1016/j.fct.2009.02.01619249334
Saleem M,Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpeneCancer LettYear: 200928510911510.1016/j.canlet.2009.04.03319464787
Kaurinovic B,Popovic M,Cebovic T,Mimika-Dukic N,Effects of Calendula officinalis L. and Taraxacum officinale Weber (Asteraceae) extracts on production of OH radicalsFresenius Environ BullYear: 200312250253
Cetkovic GS,Djilas SM,Canadanovic-Brunet JM,Tumbas VT,Antioxidant properties of marigold extractsFood Res IntYear: 20043764365010.1016/j.foodres.2004.01.010
Miliauskas G,Venskutonis PR,van Beek TA,Screening of radical scavenging activity of some medicinal and aromatic plant extractsFood ChemYear: 20048523123710.1016/j.foodchem.2003.05.007
Muley BP,Khadabadi SS,Banarase NB,Phytochemical constituents and pharmacological activities of Calendula officinalis Linn (Asteraceae)Tropical J Pharm ResYear: 20098455465
Vodnar DC,Inhibition of Listeria monocytogenes ATCC 19115 on ham steak by tea bioactive compounds incorporated into chitosan-coated plastic filmsChem Central JYear: 201267410.1186/1752-153X-6-74
Badami RC,Patil KB,Structure and occurrence of unusual fatty acids in minor seed oilProg Lipid ResYear: 1981191191537033990
Aitzetmüller K,Capillary GLC fatty acid fingerprints of seed lipids – A tool in plant chemotaxonomy?J High Resol ChromatogrYear: 19931648849010.1002/jhrc.1240160809
McLean J,Clark AH,The isolation of octadec-8:10:12-trienoic acid from marigold seed oilJ Chem SocYear: 1956I777778
Chisholm MJ,Hopkins CY,Conjugated fatty acids of Tragopogon an Calendula seed oilsCan J ChemYear: 1960382500250710.1139/v60-339
Tulloch AP,13 C Nuclear Magnetic Resonance spectroscopic analysis of seed oils containing conjugated unsaturated acidsLipidsYear: 19821780546551
Saleem M,Zaka G,Shakir N,Khan SA,Studies on marigold seed oil and seed mealFette, Seifen, AnstrichmYear: 19868817818010.1002/lipi.19860880507
Crombie L,Holloway SJ,The biosynthesis of calendic acid, octadeca-(8E, 10E, 12Z)-trienoic acid, by developing marigold seeds: origins of (E, E, Z) and (Z, E, Z) conjugated triene acids in higher plantsJ Chem Soc, Perkin TransYear: 1985124252434
Fritsche K,Hornung E,Petzsch N,Renz A,Feussner I,Isolation and characterization of a calendic acid producing (8,11)-linoleoyl desaturaseFEBS LettYear: 1999462324925310.1016/S0014-5793(99)01541-010622705
Cahoon EB,Ripp KG,Hall SE,Kinney AJ,Formation of conjugated delta8, delta10-double bonds by delta12-oleic-acid desaturase-related enzymes: biosynthetic origin of calendic acidJ Biol ChemYear: 200127642637264310.1074/jbc.M00918820011067856
Qiu X,Reed DW,Hong H,MacKenzie SL,Covello PS,Identification and analysis of a gene from Calendula officinalis encoding a fatty acid conjugasePlant PhysiolYear: 2001125284785510.1104/pp.125.2.84711161042
Reed DW,Savile C,Qiu X,Buist PH,Covello PS,Mechanism of 1,4 dehydrogenation catalyzed by a fatty acid (1,4)-desaturase of Calendula officinalisEur J BiochemYear: 2002269205024502910.1046/j.1432-1033.2002.03209.x12383261
Derksen JTP,Cuperus PF,Kolster P,Renewable resources in coatings technology: a reviewProg Org CoatYear: 1996271455310.1016/0300-9440(95)00518-8
Metzger JO,Bornscheurer U,Lipids as renewable resources: current state of chemical and biotechnological conversion and diversificationAppl Microbiol BiotechnolYear: 2006711132210.1007/s00253-006-0335-416604360
Hennessy AA,Ross RP,Devery R,Stanton C,The health promoting properties of the conjugated isomers of α-linolenic acidLipidsYear: 20114610511910.1007/s11745-010-3501-521161605
Cromack HTH,Smith JM,Calendula officinalis production potential and crop agronomy in Southern EnglandInd Crop ProdYear: 1998722322910.1016/S0926-6690(97)00052-6
Cahoon EB,Dietrich CR,Meyer K,Damude HG,Dyer JM,Kinney AJ,Conjugated fatty acids accumulate to high levels in phospholipids of metabolically engineered soybean and Arabidopsis seedsPhytochemYear: 200667121166117610.1016/j.phytochem.2006.04.013
Cahoon EB,Shockey JM,Dietrich CR,Gidda SK,Mullen RT,Dyer JM,Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid fluxCurr Opin Plant BiolYear: 20071023624410.1016/j.pbi.2007.04.00517434788
Chardigny JM,Hasselwander O,Genty M,Kraemer K,Ptock A,Sebedio JL,Effect of conjugated FA on feed intake, body composition, and liver FA in miceLipidsYear: 200338989590210.1007/s11745-003-1142-514584596
Suzuki R,Noguchi R,Ota T,Abe M,Miyashita K,Kawada T,Cytotoxic effect of conjugated trienoic fatty acids on mouse tumor and human monocytic leukemia cellsLipidsYear: 200136547748210.1007/s11745-001-0746-011432460
Yasui Y,Hosokawa M,Kohno H,Tanaka T,Miyashita K,Growth inhibition and apoptosis induction by all-trans-conjugated linolenic acids on human colon cancer cellsAnticancer ResYear: 2006263A1855186016827117
Bhaskar N,Masashi H,Yumiko Y,Rikako S,Hiroyuki K,Takuji T,Kazuo M,Chemopreventive effects of conjugated linolenic acids (CLN) occurring in plant seed oilsCurr Nutr Food SciYear: 20073430931810.2174/1573401310703040309
Ul'chenko NT,Glushenkova AI,Mukhamedova KS,Lipids of Calendula officinalisChem Nat CompdYear: 199834327227410.1007/BF02282400
Pintea A,Dulf F,Bele C,Andrei S,Fatty acids distribution in the lipid fractions of Calendula officinalis L. seeds oilChem ListyYear: 200810215749750
Özgül-Yücel S,Determination of conjugated linolenic acid content of selected oil seeds grown in TurkeyJ Am Oil Chem SocYear: 2005821289389710.1007/s11746-005-1161-7
Angelini LG,Moscheni E,Colonna G,Belloni P,Bonari E,Variation in agronomic characteristics and seed oil composition of new oilseed crops in central ItalyInd Crops ProdYear: 1997631332310.1016/S0926-6690(97)00022-8
Yang BR,Kallio HP,Fatty acid composition of lipids in sea buckthorn (Hippophaë rhamnoides L.) berries of different originsJ Agric Food ChemYear: 2001491939194710.1021/jf001059s11308350
Muuse BG,Cuperus FP,Derksen JTP,Composition and physical properties of oils from new oil seed cropsInd Crop ProdYear: 199211576510.1016/0926-6690(92)90046-X
Zlatanov MD,Lipid composition of Bulgarian chokeberry, black currant and rose hip seed oilsJ Sci Food AgricYear: 1999791620162410.1002/(SICI)1097-0010(199909)79:12<1620::AID-JSFA410>3.0.CO;2-G
Kallio H,Yang B,Peippo P,Tahvonen R,Pan R,Triacylglycerols, glycerophospholipids, tocopherols, and tocotrienols in berries and seeds of two subspecies (ssp. sinensis and mongolica) of sea buckthorn (Hippophaë rhamnoides)J Agric Food ChemYear: 2002503004300910.1021/jf011556o11982433
Yang B,Koponen J,Tahvonen R,Kallio H,Plant sterols in seeds of two species of Vaccinium (V. myrtillus and V. vitis-idaea) naturally distributed in FinlandEur Food Res TechnolYear: 20032163438
Lee SB,Jung SJ,Go YS,Kim HU,Kim JK,Cho HJ,Park OK,Suh MC,Two Arabidopsis 3-ketoacyl CoA synthase genes, KCS20 and KCS2/DAISY, are functionally redundant in cuticular wax and root suberin biosynthesis, but differentially controlled by osmotic stressPlant JYear: 20096046247510.1111/j.1365-313X.2009.03973.x19619160
Baciu AD,Mihalte L,Sestras A,Sestras R,Variability of decorative traits, response to the Aphis fabae attack and RAPD diversity in different genotypes of CalendulaNot. Bot. Hort. Agrobot. ClujYear: 2010383265270
Diabetes and Nutrition Study Group of the Spanish Diabetes Association (GSEDNu)Diabetes nutrition and complications trial: adherence to the ADA nutritional recommendations, targets of metabolic control, and onset of diabetes complications. A 7-year, prospective, population-based, observational multicenter studyJ Diabetes ComplicatYear: 20062036136617070439
Kang MJ,Shin MS,Park JN,Lee SS,The effects of polyunsaturated: saturated fatty acids ratios and peroxidisability index values of dietary fats on serum lipid profiles and hepatic enzyme activities in ratsBrit J NutrYear: 20059452653210.1079/BJN2005152316197576
Guo Z,Miura K,Turin TC,Hozawa A,Okuda N,Okamura T,Saitoh S,Sakata K,Nakagawa H,Okayama A,Yoshita K,Kadowaki T,Choudhury SR,Nakamura Y,Rodriguez BL,Curb DJ,Elliott P,Stamler J,Ueshima H,Relationship of the polyunsaturated to saturated fatty acid ratio to cardiovascular risk factors and metabolic syndrome in Japanese: the INTERLIPID studyJ Atheroscler ThrombYear: 20101777778410.5551/jat.413520351467
Dulf FV,Andrei S,Bunea A,Socaciu C,Fatty acid and phytosterol contents of some Romanian wild and cultivated berry pomacesChem PapYear: 20126610925934
Dulf FV,Fatty acids in berry lipids of six sea buckthorn (Hippophae rhamnoides L., subspecies carpatica) cultivars grown in RomaniaChem Cent JYear: 2012610610.1186/1752-153X-6-10622995716
Kramer JKG,Cruz-Hernandez C,Deng Z,Zhou J,Jahreis G,Dugan MER,Analysis of conjugated linoleic acid and trans 18:1 isomers in synthetic and animal productsAm J Clin NutrYear: 2004791137S1145S15159247
Christie WW,Christie W, Glasgow Preparation of methyl ester and other derivativesGas Chromatography and Lipids. A Practical GuideYear: 1989Great Britain: The Oily Press3647

Figures

[Figure ID: F1]
Figure 1 

The oil content of Pot marigold (Calendula officinalis L.) seeds. CO1- CO11, pot marigold (Calendula officinalis L.) genotypes. Results are given as mean ± SD (n = 3); * - significant difference, p < 0.05 (using "Kruskal-Wallis non-parametric test" followed by "Dunn's Multiple Comparison Test").



[Figure ID: F2]
Figure 2 

GC-MS chromatogram of FAMEs in the TLs of Calendula officinalis L. (CO2: cv. Prolifera nr. 214) seeds analysed with a BPx- 70 capillary column. Peaks: (1) lauric (12:0), (2) myristic (14:0), (3) pentadecanoic (15:0), (4) cis-7 hexadecenoic [16:1 (n-9)], (5) palmitoleic [16:1 (n-7)], (6) palmitic (16:0), (7) margaric (17:0), (8) linoleic [18:2 (n-6)], (9) oleic [18:1 (n-9)], (10) elaidic [18:1 (9 t) (n-9)], (11) linoelaidicic [18:2 (9 t,12 t) (n-6)], (12) stearic (18:0), (13) α- linolenic [18:3 (n-3)], (14) calendic [18:3 (8 t, 10 t, 12c) (n-6)], (15) gondoic [20:1 (n-9)], (16) β- calendic [18:3 (8 t, 10 t, 12 t) (n-6)], (17) arachidic (20:0), (18) 9- hydroxy- trans-10, cis-12 octadecadienic (9- HODE), (19) behenic (22:0) acids.



[Figure ID: F3]
Figure 3 

Comparative representation of fatty acid classes from total lipids of different genotypes of pot marigold (Calendula officinalis L.) seed oils. CO1- CO11, pot marigold (Calendula officinalis L.) genotypes. Values are mean ± SD of three samples, analyzed individually in triplicate (n = 3x3). Values with different letters (a-e) are significantly different (p < 0.05), using ANOVA "Tukey's Multiple Comparison Test". SFAs- saturated fatty acids, MUFAs- monounsaturated fatty acids, PUFAs- polyunsaturated fatty acids, CLNAs- conjugated linolenic acids.



Tables
[TableWrap ID: T1] Table 1 

Fatty acid composition (%) in total lipids and individual lipid classes of different genotypes of pot marigold seed oils


Species
Fatty acids (%w/w of total fatty acids)
  12:0 14:0 15:0 16:1 (n-9) 16:1 (n-7) 16:0 17:0 18:2 (n-6) 18:1 (n-9) 18:1 (9t) (n-9) 18:2 (9t,12t) (n-6) 18:0 18:3 (n-3) 18:3 (8t,10t,12c) (n-6) 20:1 (n-9) 18:3 (8t,10t,12t), (n-6) 20:0 9-HODE 22:0
CO1
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.02
0.18
0.01
0.03
0.08
4.05
0.03
30.05
4.99
0.51
tr
1.87
0.09
55.93
0.14
0.85
0.36
0.63
0.18
TAG
nd
0.15
0.08
0.03
0.08
5.62
tr
29.18
5.76
0.50
nd
2.62
0.15
54.67
0.10
0.60
0.46
nd
nd
PL
0.64
3.88
0.27
0.31
0.15
17.08
nd
60.78
6.49
0.70
tr
3.72
nd
4.29
tr
0.43
0.59
tr
0.67
SE
0.62
2.86
nd
tr
nd
20.02
nd
34.91
11.02
0.12
nd
2.53
nd
4.64
nd
nd
2.45
nd
20.83
CO2
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.03
0.30
0.01
0.03
0.07
4.33
0.04
28.50
4.49
0.53
0.02
1.84
0.10
57.22
0.16
0.77
0.42
0.89
0.25
TAG
nd
0.13
0.02
0.04
0.18
7.98
0.04
25.10
9.10
1.10
nd
3.95
0.09
50.03
0.47
0.70
1.07
nd
nd
PL
0.41
4.44
0.15
0.16
0.08
20.61
0.17
55.02
7.04
1.01
1.06
3.94
nd
3.98
0.07
0.40
0.61
0.17
0.68
SE
0.91
3.05
nd
0.39
nd
20.23
nd
33.75
10.32
0.14
nd
2.97
nd
4.94
nd
nd
2.53
nd
20.77
CO3
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.01
0.15
0.01
0.03
0.07
3.93
0.02
31.79
5.06
0.54
0.06
1.73
0.10
54.07
0.15
0.79
0.38
0.95
0.17
TAG
nd
0.14
0.02
0.04
0.18
10.21
0.07
24.30
10.02
0.73
nd
4.08
0.10
48.53
0.45
0.53
0.60
nd
nd
PL
0.17
2.45
0.12
0.13
0.12
17.66
0.15
61.33
6.88
1.13
0.76
3.43
nd
3.86
tr
0.46
0.50
0.13
0.72
SE
0.72
2.53
nd
0.33
nd
22.77
nd
32.34
12.00
0.20
nd
2.93
nd
2.69
nd
nd
2.49
nd
21.00
CO4
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.02
0.19
0.01
0.04
0.06
4.55
0.02
28.52
4.44
0.41
0.01
1.77
0.13
57.63
0.14
0.91
0.35
0.66
0.12
TAG
nd
0.15
0.03
0.08
0.18
8.59
0.04
23.32
10.79
1.20
nd
4.20
0.08
49.02
0.53
0.51
1.28
nd
nd
PL
0.22
2.29
0.12
0.26
0.12
16.55
0.16
61.15
8.13
1.30
0.74
3.18
nd
4.19
0.10
0.34
0.46
0.16
0.53
SE
1.24
2.05
0.38
0.78
nd
21.78
nd
32.41
11.80
1.53
nd
3.51
nd
3.93
nd
nd
2.68
nd
17.91
CO5
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.02
0.22
0.01
0.03
0.07
4.22
0.03
31.47
6.19
0.55
0.07
1.88
0.11
52.52
0.15
0.83
0.40
0.98
0.25
TAG
nd
0.15
0.02
0.05
0.25
8.15
0.04
24.75
13.27
1.15
nd
4.24
0.07
46.00
0.58
0.38
0.90
nd
nd
PL
0.26
3.23
0.16
0.17
0.12
20.31
0.19
57.96
7.69
1.20
0.77
3.68
nd
2.66
0.04
0.16
0.59
0.11
0.70
SE
1.01
2.11
nd
tr
nd
19.70
nd
33.52
10.31
0.37
nd
4.65
nd
4.60
nd
nd
2.80
nd
20.93
CO6
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.02
0.21
0.02
0.04
0.06
4.48
0.04
29.49
5.26
0.55
0.02
1.83
0.09
55.80
0.13
0.66
0.34
0.89
0.07
TAG
nd
0.16
0.02
0.07
0.22
9.55
0.06
22.60
12.01
1.34
nd
4.42
0.06
47.85
0.48
0.33
0.83
nd
nd
PL
0.35
2.83
0.21
0.18
0.10
20.14
0.21
59.04
7.51
1.24
0.61
3.72
nd
2.61
tr
0.13
0.59
0.06
0.47
SE
1.59
2.90
nd
tr
nd
20.37
nd
32.33
12.29
0.17
nd
3.97
nd
3.75
nd
nd
2.85
nd
19.78
CO7
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.02
0.33
0.02
0.03
0.08
4.54
0.04
30.26
6.04
0.57
1.66
1.84
0.08
53.17
tr
0.46
0.38
0.38
0.11
TAG
nd
0.16
0.02
0.10
0.22
8.62
0.08
24.62
12.65
1.13
nd
4.03
0.08
46.46
0.57
0.44
0.82
nd
nd
PL
0.29
2.74
0.13
0.16
0.14
19.90
0.22
60.46
7.20
1.13
tr
3.33
nd
2.77
0.11
0.13
0.53
0.11
0.65
SE
2.19
2.80
nd
0.71
nd
18.31
nd
33.89
10.83
0.22
nd
3.30
nd
3.77
nd
nd
3.32
nd
20.66
CO8
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.05
0.39
0.01
0.02
0.11
4.11
0.03
31.86
6.25
0.49
1.80
1.99
0.11
51.47
0.02
0.48
0.41
0.24
0.17
TAG
nd
0.16
0.02
0.05
0.29
8.26
0.05
23.37
14.32
1.11
nd
4.77
0.06
45.73
0.53
0.43
0.85
nd
nd
PL
0.28
2.34
0.16
0.25
0.14
19.37
0.22
58.97
8.82
1.22
0.24
3.63
nd
2.91
tr
0.19
0.54
0.13
0.59
SE
1.75
3.14
nd
1.51
nd
23.77
nd
27.46
10.07
0.48
nd
3.03
nd
4.74
nd
nd
3.76
nd
20.29
CO9
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.03
0.19
0.02
0.02
0.10
3.98
0.05
30.81
4.98
0.53
1.34
1.87
0.12
54.21
tr
0.58
0.39
0.27
0.51
TAG
nd
0.11
0.03
0.04
0.25
6.49
0.07
27.68
8.94
1.01
nd
3.59
0.10
50.06
0.47
0.53
0.63
nd
nd
PL
0.19
1.14
0.16
0.26
0.23
20.82
0.24
59.34
7.28
1.22
0.88
3.58
nd
2.95
0.05
0.17
0.62
0.10
0.77
SE
1.50
3.58
nd
1.05
nd
20.42
nd
32.38
9.68
0.24
nd
2.88
nd
3.69
nd
nd
3.37
nd
21.21
CO10
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.02
0.16
0.02
0.02
0.12
3.86
0.03
30.99
4.97
0.53
1.71
1.87
0.11
53.88
tr
0.65
0.36
0.29
0.41
TAG
nd
0.08
0.01
0.05
0.31
6.99
0.05
27.93
9.09
1.06
nd
3.76
0.07
49.18
0.43
0.41
0.58
nd
nd
PL
0.12
0.95
0.15
0.25
0.25
20.28
0.27
61.51
6.98
1.16
0.35
3.51
nd
2.84
0.05
0.20
0.55
0.09
0.49
SE
1.86
3.31
nd
tr
nd
20.83
nd
32.16
10.11
0.23
nd
3.47
nd
3.43
tr
nd
3.62
nd
20.98
CO11
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
TL
0.03
0.38
0.02
0.04
0.05
4.43
0.04
30.32
4.78
0.56
0.62
1.75
0.11
55.32
0.05
0.61
0.39
0.21
0.31
TAG
nd
0.13
0.02
0.07
0.21
7.79
0.04
27.44
8.36
1.15
nd
3.43
0.09
49.85
0.39
0.48
0.55
nd
nd
PL
0.33
2.36
0.15
0.45
0.09
20.98
0.25
59.55
7.71
1.24
tr
3.31
nd
2.34
tr
0.13
0.51
0.04
0.56
SE 1.57 3.36 nd tr nd 20.93 nd 31.84 10.88 0.19 nd 3.26 nd 3.19 nd nd 3.61 nd 21.17

The values represent the means of three samples, analyzed individually in triplicate (n = 3x3).

CO1- CO11, pot marigold (Calendula officinalis L.) genotypes.

TL- total lipids, TAG- triacylglycerols, PL- polar lipids, SE- sterol esters, nd- not detected, tr- trace.


[TableWrap ID: T2] Table 2 

The composition (%) of fatty acid classes in total lipids and major lipid fractions from different genotypes of pot marigold seed oils


Species Fatty acids (%w/w of total fatty acids)
∑ SFAs
∑ MUFAs
∑ PUFAs
∑ VLCSFAs (≥20C)
∑CLNAs
PUFAs/SFAs
Mean SD Mean SD Mean SD Mean SD Mean SD
CO1
 
 
 
 
 
 
 
 
 
 
 
TL
6.70d
0.25
5.75d
0.18
86.83a
2.25
0.54b
0.02
56.78a
1.76
12.96a
TAG
8.93c
0.35
6.47c
0.20
84.45a
2.30
0.46b
0.03
55.27a
1.55
9.46b
PL
26.85b
1.11
7.65b
0.22
65.50b
1.85
1.26b
0.04
4.72b
0.15
2.44c
SE
49.31a
1.68
11.14a
0.32
39.55c
1.68
23.28a
1.05
4.64b
0.12
0.80d
CO2
 
 
 
 
 
 
 
 
 
 
 
TL
7.23d
0.22
5.27c
0.15
86.52a
2.30
0.67b
0.03
57.99a
1.65
11.97a
TAG
13.19c
0.30
10.89a
0.22
75.83b
2.27
1.07b
0.03
50.73b
1.38
5.75b
PL
31.01b
1.10
8.36b
0.18
60.46c
2.10
1.29b
0.05
4.38c
0.12
1.95c
SE
50.46a
1.80
10.85a
0.20
38.69d
1.50
23.30a
0.70
4.94c
0.15
0.77d
CO3
 
 
 
 
 
 
 
 
 
 
 
TL
6.39d
0.19
5.85d
0.16
86.70a
2.38
0.54b
0.03
54.85a
1.32
13.56a
TAG
15.12c
0.35
11.42b
0.25
73.36b
1.95
0.60b
0.03
49.06b
1.75
4.85b
PL
25.20b
0.80
8.26c
0.18
66.41c
1.55
1.22b
0.04
4.32c
0.11
2.64c
SE
52.44a
1.60
12.53a
0.30
35.03d
1.15
23.49a
0.56
2.69c
0.10
0.67d
CO4
 
 
 
 
 
 
 
 
 
 
 
TL
7.04d
0.16
5.09d
0.15
87.08a
2.42
0.47b
0.02
58.54a
1.58
12.37a
TAG
14.29c
0.31
12.78b
0.23
72.85b
2.10
1.28b
0.03
49.53b
1.65
5.10b
PL
23.51b
0.62
9.91c
0.22
66.42c
1.60
0.99b
0.03
4.53c
0.14
2.83c
SE
49.55a
1.55
14.11a
0.35
36.34d
1.11
20.59a
0.50
3.93c
0.11
0.73d
CO5
 
 
 
 
 
 
 
 
 
 
 
TL
7.03d
0.17
6.99d
0.15
84.89a
2.30
0.66b
0.04
53.35a
1.25
12.08a
TAG
13.50c
0.28
15.30a
0.32
71.13b
2.00
0.90b
0.04
46.38b
1.60
5.27b
PL
29.12b
0.88
9.22c
0.20
61.55c
1.50
1.29b
0.03
2.82c
0.12
2.11c
SE
51.20a
1.65
10.68b
0.25
38.12d
1.20
23.73a
0.65
4.60c
0.14
0.74d
CO6
 
 
 
 
 
 
 
 
 
 
 
TL
7.01d
0.20
6.03d
0.18
85.98a
2.35
0.41b
0.02
56.47a
1.35
12.26a
TAG
15.04c
0.32
14.12a
0.35
70.78b
2.10
0.83b
0.03
48.18b
1.62
4.71b
PL
28.52b
0.88
9.03c
0.25
62.39c
1.60
1.06b
0.02
2.74c
0.08
2.19c
SE
51.46a
1.70
12.46b
0.34
36.08d
1.15
22.63a
0.60
3.75c
0.09
0.70d
CO7
 
 
 
 
 
 
 
 
 
 
 
TL
7.27d
0.22
6.72d
0.20
85.55a
2.40
0.48b
0.02
53.63a
1.30
11.77a
TAG
13.73c
0.30
14.67a
0.33
71.52b
2.15
0.82b
0.03
46.90b
1.25
5.21b
PL
27.79b
0.80
8.74c
0.24
63.36c
1.70
1.18b
0.03
2.90c
0.10
2.28c
SE
50.58a
1.48
11.76b
0.20
37.66d
1.10
23.98a
0.70
3.77c
0.15
0.74d
CO8
 
 
 
 
 
 
 
 
 
 
 
TL
7.15d
0.17
6.90d
0.18
85.61a
2.29
0.57b
0.03
51.95a
1.38
11.98a
TAG
14.11c
0.40
16.30a
0.40
69.53b
1.98
0.85b
0.04
46.16b
1.30
4.93b
PL
27.13b
0.82
10.43c
0.28
62.31c
1.50
1.13b
0.03
3.10c
0.11
2.30c
SE
55.74a
1.85
12.06b
0.32
32.20d
0.88
24.05a
0.75
4.74c
0.14
0.58d
CO9
 
 
 
 
 
 
 
 
 
 
 
TL
7.04d
0.14
5.63c
0.16
86.94a
2.34
0.90b
0.03
54.80a
1.38
12.35a
TAG
10.92c
0.30
10.71a
0.31
78.27b
2.15
0.63b
0.04
50.59b
1.28
7.17b
PL
27.52b
0.72
9.04b
0.28
63.34c
1.52
1.39b
0.02
3.12c
0.06
2.30c
SE
52.96a
1.60
10.97a
0.30
36.07d
1.05
24.58a
0.65
3.69c
0.07
0.68d
CO10
 
 
 
 
 
 
 
 
 
 
 
TL
6.74d
0.16
5.64c
0.14
87.22a
2.55
0.77b
0.03
54.52a
1.35
12.95a
TAG
11.47c
0.31
10.94a
0.28
77.52b
2.20
0.58b
0.03
49.59b
1.18
6.76b
PL
26.32b
0.72
8.69b
0.25
64.90c
1.55
1.04b
0.02
3.04c
0.08
2.47c
SE
54.07a
1.65
10.34a
0.26
35.59d
0.95
24.60a
0.68
3.43c
0.08
0.66d
CO11
 
 
 
 
 
 
 
 
 
 
 
TL
7.34d
0.18
5.48d
0.12
86.87a
2.60
0.70b
0.03
55.93a
1.40
11.83a
TAG
11.96c
0.30
10.18b
0.25
77.77b
2.25
0.55b
0.04
50.33b
1.20
6.50b
PL
28.45b
0.70
9.49c
0.22
62.02c
1.52
1.07b
0.03
2.47c
0.08
2.18c
SE 53.90a 1.75 11.07a 0.27 35.03d 0.90 24.78a 0.80 3.19c 0.09 0.65d

Values are given as mean ± SD of three samples, analyzed individually in triplicate (n = 3x3).

Means in the same column followed by different subscript letters indicate significant differences (p < 0.05) among lipid classes of each genotype of pot marigold (ANOVA "Tukey's Multiple Comparison Test"). TL - total lipids, TAG- triacylglycerols, PL- polar lipids, SE- sterol esters. SFAs- saturated fatty acids, MUFAs- monounsaturated fatty acids, PUFAs- polyunsaturated fatty acids, VLCSFAs- very long chain saturated fatty acids, ∑CLNAs [18:3 (8trans, 10trans, 12cis) + 18:3 (8trans,10trans,12trans)] - conjugated linolenic acids.


[TableWrap ID: T3] Table 3 

Genotypes of Calendula officinalis L. (CO) evaluated


Samples Genotypes Sources
CO1
C. officinalis L. D.a
Humboldt-Universität zu Berlin, Institut für Biologie, Germany
CO2
cv. Prolifera nr. 214
Botanische Garten der Universität Göttingen, Germany
CO3
Bon-Bon Orange
National Botanic Garden of Latvia, Salaspils, Latvia
CO4
Bon-Bon Mix’
Hortus Botanicus Fominianus, Kiev, Ukraine
CO5
cv. Radio
Ökologisch-Botanischer Garten der Universität Bayreuth, Germany
CO6
C. officinalis L. PL
Hortus Farmacognosticus Academiae Medicinalis, Lublin, Poland
CO7
C. officinalis L. I
Instituto di Botanica e Orto Botanico Pierino Scaramella, Italy
CO8
cv. Prycosnovjenie
National Botanical Gardens Timirjazevska, Kiev, Ukraine
CO9
cv. Pacific Beauty
National Botanical Gardens Timirjazevska, Kiev, Ukraine
CO10
cv. Zelenoye Serdtse
National Botanical Gardens Timirjazevska, Kiev, Ukraine
CO11 cv. Plamen Masarykova Univerzita Brno, Czech Republic


Article Categories:
  • Research Article

Keywords: Calendula officinalis L., Conjugated linolenic acids, Pot marigold, Seed oils, Fatty acids, Polar lipids, Triacylglycerols, Sterol esters, GC-MS.

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