Document Detail

Fatty acid biosynthesis is involved in solvent tolerance in Pseudomonas putida DOT-T1E.
MedLine Citation:
PMID:  15008818     Owner:  NLM     Status:  MEDLINE    
The unusual tolerance of Pseudomonas putida DOT-T1E to toluene is based on the extrusion of this solvent by constitutive and inducible efflux pumps and rigidification of its membranes via phospholipid alterations. Pseudomonas putida DOT-T1E-109 is a solvent-sensitive mutant. Mutant cells were less efficient in solvent extrusion than the wild-type cells, as shown by the limited efflux of 14C-1,2,4-trichlorobenzene from the cell membranes, despite the fact that the efflux pumps are overexpressed as a result of increased expression of the ttgDEF and ttgGHI efflux pump operons. This limitation could be the result of alterations in the outer membrane because the mutant cells released more beta-lactamase to the external medium than the wild-type cells. The mutant P. putida DOT-T1E-109 showed negligible synthesis of fatty acids in the presence of sublethal concentrations of toluene as revealed by analysis of 13CH3-13COOH incorporation into fatty acids. In contrast, the mutant strain in the absence of solvents, and the wild-type strain, both in the presence and in the absence of toluene, incorporated 13CH3-13COOH at a high rate into de novo synthesized lipids. The mutation in P. putida DOT-T1E-109 increases sensitivity to the solvent because of a limited efflux of the solvent from the cell membranes with the concomitant inhibition of fatty acid biosynthesis.
Ana Segura; Estrella Duque; Antonia Rojas; Patricia Godoy; Antonio Delgado; Ana Hurtado; John Cronan; Juan-Luis Ramos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental microbiology     Volume:  6     ISSN:  1462-2912     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-10     Completed Date:  2004-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  416-23     Citation Subset:  IM    
Department of Biochemistry and Molecular and Cellular Biology of Plants, CSIC-Estación Experimental del Zaidín, E-18008 Granada, Spain.
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MeSH Terms
Fatty Acids / biosynthesis*
Membrane Lipids / metabolism
Membrane Transport Proteins / metabolism
Pseudomonas putida / cytology,  genetics,  metabolism*
Solvents / chemistry,  metabolism*
Reg. No./Substance:
0/Fatty Acids; 0/Membrane Lipids; 0/Membrane Transport Proteins; 0/Solvents

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