| Fatty acid binding protein 4 expression marks a population of adipocyte progenitors in white and brown adipose tissues. | |
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MedLine Citation:
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PMID: 23047894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adipose tissues regulate metabolism, reproduction, and life span. The development and growth of adipose tissue are due to increases of both adipocyte cell size and cell number; the latter is mediated by adipocyte progenitors. Various markers have been used to identify either adipocyte progenitors or mature adipocytes. The fatty acid binding protein 4 (FABP4), commonly known as adipocyte protein 2 (aP2), has been extensively used as a marker for differentiated adipocytes. However, whether aP2 is expressed in adipogenic progenitors is controversial. Using Cre/LoxP-based cell lineage tracing in mice, we have identified a population of aP2-expressing progenitors in the stromal vascular fraction (SVF) of both white and brown adipose tissues. The aP2-lineage progenitors reside in the adipose stem cell niche and express adipocyte progenitor markers, including CD34, Sca1, Dlk1, and PDGFRα. When isolated and grown in culture, the aP2-expressing SVF cells proliferate and differentiate into adipocytes upon induction. Conversely, ablation of the aP2 lineage greatly reduces the adipogenic potential of SVF cells. When grafted into wild-type mice, the aP2-lineage progenitors give rise to adipose depots in recipient mice. Therefore, the expression of aP2 is not limited to mature adipocytes, but also marks a pool of undifferentiated progenitors associated with the vasculature of adipose tissues. Our finding adds to the repertoire of adipose progenitor markers and points to a new regulator of adipose plasticity. |
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Authors:
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Tizhong Shan; Weiyi Liu; Shihuan Kuang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-10-09 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 27 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-01-03 Completed Date: 2013-03-07 Revised Date: 2013-05-10 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 277-87 Citation Subset: IM |
Affiliation:
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Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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metabolism* Adipose Tissue / cytology, metabolism* Adipose Tissue, Brown / cytology, metabolism* Animals Cell Proliferation Cell Separation Cells, Cultured Fatty Acid-Binding Proteins Flow Cytometry Mice Real-Time Polymerase Chain Reaction Stem Cells / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 AR060652/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/FABP4 protein, human; 0/Fatty Acid-Binding Proteins |
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