Document Detail


Fatty acid binding protein 4 expression marks a population of adipocyte progenitors in white and brown adipose tissues.
MedLine Citation:
PMID:  23047894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adipose tissues regulate metabolism, reproduction, and life span. The development and growth of adipose tissue are due to increases of both adipocyte cell size and cell number; the latter is mediated by adipocyte progenitors. Various markers have been used to identify either adipocyte progenitors or mature adipocytes. The fatty acid binding protein 4 (FABP4), commonly known as adipocyte protein 2 (aP2), has been extensively used as a marker for differentiated adipocytes. However, whether aP2 is expressed in adipogenic progenitors is controversial. Using Cre/LoxP-based cell lineage tracing in mice, we have identified a population of aP2-expressing progenitors in the stromal vascular fraction (SVF) of both white and brown adipose tissues. The aP2-lineage progenitors reside in the adipose stem cell niche and express adipocyte progenitor markers, including CD34, Sca1, Dlk1, and PDGFRα. When isolated and grown in culture, the aP2-expressing SVF cells proliferate and differentiate into adipocytes upon induction. Conversely, ablation of the aP2 lineage greatly reduces the adipogenic potential of SVF cells. When grafted into wild-type mice, the aP2-lineage progenitors give rise to adipose depots in recipient mice. Therefore, the expression of aP2 is not limited to mature adipocytes, but also marks a pool of undifferentiated progenitors associated with the vasculature of adipose tissues. Our finding adds to the repertoire of adipose progenitor markers and points to a new regulator of adipose plasticity.
Authors:
Tizhong Shan; Weiyi Liu; Shihuan Kuang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-09
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  27     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-03     Completed Date:  2013-03-07     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  277-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / metabolism*
Adipose Tissue / cytology,  metabolism*
Adipose Tissue, Brown / cytology,  metabolism*
Animals
Cell Proliferation
Cell Separation
Cells, Cultured
Fatty Acid-Binding Proteins
Flow Cytometry
Mice
Real-Time Polymerase Chain Reaction
Stem Cells / metabolism
Grant Support
ID/Acronym/Agency:
R01 AR060652/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/FABP4 protein, human; 0/Fatty Acid-Binding Proteins
Comments/Corrections

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