Document Detail

Fatty acid alcohol ester-synthesizing activity of lipoprotein lipase.
MedLine Citation:
PMID:  10578059     Owner:  NLM     Status:  MEDLINE    
The fatty acid alcohol ester-synthesizing activity of lipoprotein lipase (LPL) was characterized using bovine milk LPL. Synthesizing activities were determined in an aqueous medium using oleic acid or trioleylglycerol as the acyl donor and equimolar amounts of long-chain alcohols as the acyl acceptor. When oleic acid and hexadecanol emulsified with gum arabic were incubated with LPL, palmityl oleate was synthesized, in a time- and dose-dependent manner. Apo-very low density lipoprotein (apoVLDL) stimulated LPL-catalyzed palmityl oleate synthesis. The apparent equilibrium ratio of fatty acid alcohol ester/oleic acid was estimated using a high concentration of LPL and a long (20 h) incubation period. The equilibrium ratio was affected by the incubation pH and the alcohol chain length. When the incubation pH was below pH 7.0 and long chain fatty acyl alcohols were used as substrates, the fatty acid alcohol ester/free fatty acid equilibrium ratio favored ester formation, with an apparent equilibrium ratio of fatty acid alcohol ester/fatty acid of about 0.9/0.1. The equilibrium ratio decreased sharply at alkaline pH (above pH 8.0). The ratio also decreased when fatty alcohols with acyl chains shorter than dodecanol were used. When a trioleoylglycerol/fatty acyl alcohol emulsion was incubated with LPL, fatty acid alcohol esters were synthesized in a dose- and time-dependent fashion. Fatty acid alcohol esters were easily synthesized from trioleoylglycerol when fatty alcohols with acyl chains longer than dodecanol were used, but synthesis was decreased with fatty alcohols with acyl chain lengths shorter than decanol, and little synthesizing activity was detected with shorter-chain fatty alcohols such as butanol or ethanol.
T Tsujita; M Sumiyoshi; H Okuda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  126     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-03-28     Completed Date:  2000-03-28     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1074-9     Citation Subset:  IM    
Central Research Laboratory School of Medicine, Ehime University, Shigenobu, Onsen-gun, Ehime, 791-0295, Japan.
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MeSH Terms
Apolipoproteins / metabolism
Fatty Acids, Unsaturated / metabolism
Fatty Alcohols / metabolism*
Hydrogen-Ion Concentration
Lipoprotein Lipase / metabolism*
Lipoproteins, VLDL / metabolism
Milk / enzymology
Oleic Acid / metabolism
Triglycerides / metabolism
Reg. No./Substance:
0/Apolipoproteins; 0/Emulsions; 0/Fatty Acids, Unsaturated; 0/Fatty Alcohols; 0/Lipoproteins, VLDL; 0/Triglycerides; 0/palmityl oleate; 112-80-1/Oleic Acid; EC Lipase

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