Document Detail

Fatty Acyl-AMP Ligase Involvement in the Production of Alkylresorcylic Acid by a Myxococcus xanthus Type III Polyketide Synthase.
MedLine Citation:
PMID:  21815236     Owner:  NLM     Status:  Publisher    
Fatty acyl-AMP ligases (FAALs) activate fatty acids as acyladenylates, and subsequently catalyze their transfer onto the acyl carrier proteins (ACPs) of polyketide synthases (PKSs) or nonribosomal peptide synthetases to produce lipidic metabolites. Myxococcus xanthus contains a polyketide biosynthesis gene cluster in which putative FAAL (FtpD) and ACP (FtpC) genes are located close to a type III PKS (FtpA) gene. Here we describe the characterization of these three proteins in vitro. FtpD adenylated stearic acid and produced stearoyl-FtpC. The stearoyl moiety was then transferred to FtpA. When extender substrates (malonyl-CoA and methylmalonyl-CoA) were added to the reaction, the alkylresorcinol 5-heptadecyl-4-methyl-benzene-1,3-diol was synthesized. Further in vitro analysis indicated that FtpA produces an alkylresorcylic acid as the direct product, and that this decarboxylates to alkylresorcinol nonenzymatically. This is the first report of a FAAL supplying a long-chain fatty acyl-ACP starter substrate to a type III PKS.
Takayuki Hayashi; Yuta Kitamura; Nobutaka Funa; Yasuo Ohnishi; Sueharu Horinouchi
Related Documents :
7143816 - A case of cerebrotendinous xanthomatosis: effects of ursodeoxycholic acid administratio...
9058626 - Soluble intercellular adhesion molecule-1 in primary biliary cirrhosis: effect of ursod...
7484996 - Combined bile acid therapy is more effective on biliary lipids and dissolution rates th...
7276746 - Thin-layer chromatographic separation of conjugates of ursodeoxycholic acid from those ...
6133786 - The effect of initiated involution on enzyme activity and substrate uptake by the mamma...
18346306 - Physiological concentrations of short-chain fatty acids immediately suppress colonic ep...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-4
Journal Detail:
Title:  Chembiochem : a European journal of chemical biology     Volume:  -     ISSN:  1439-7633     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100937360     Medline TA:  Chembiochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8567 (Japan).
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Dutch Cannabis Dependence (CanDep) study on the course of frequent cannabis use and dependence: ...
Next Document:  Novel low-cost prostate resection trainer-description and preliminary evaluation.