Document Detail


Fatty acids regulate endothelial lipase and inflammatory markers in macrophages and in mouse aorta: a role for PPARγ.
MedLine Citation:
PMID:  23042820     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Macrophage endothelial lipase (EL) is associated with increased atherosclerosis and inflammation. Because of their anti-inflammatory properties we hypothesized that n-3 fatty acids, in contrast to saturated fatty acids, would lower macrophages and arterial EL and inflammatory markers.
METHODS AND RESULTS: Murine J774 and peritoneal macrophages were incubated with eicosapentaenoic acid or palmitic acid in the presence or absence of lipopolysaccaride (LPS). LPS increased EL mRNA and protein. Palmitic acid alone or with LPS dose-dependently increased EL mRNA and protein. In contrast, eicosapentaenoic acid dose-dependently abrogated effects of LPS or palmitic acid on increasing EL expression. EL expression closely linked to peroxisome proliferator activated receptor (PPAR)γ expression. Eicosapentaenoic acid blocked rosiglitazone (a PPARγ agonist)-mediated EL activation and GW9662 (a PPARγ antagonist)-blocked palmitic acid-mediated EL stimulation. Eicosapentaenoic acid alone or with LPS blunted LPS-mediated stimulation of macrophage proinflammatory interleukin-6, interleukin-12p40, and toll-like receptor-4 mRNA and increased anti-inflammatory interleukin-10 and mannose receptor mRNA. In vivo studies in low density lipoprotein receptor knockout mice showed that high saturated fat rich diets, but not n-3 diets, increased arterial EL, PPARγ, and proinflammatory cytokine mRNA.
CONCLUSIONS: n-3 fatty acids, in contrast to saturated fatty acids, decrease EL in parallel with modulating pro- and anti-inflammatory markers, and these effects on EL link to PPARγ.
Authors:
Un Ju Jung; Claudia Torrejon; Chuchun L Chang; Hiroko Hamai; Tilla S Worgall; Richard J Deckelbaum
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-04
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  32     ISSN:  1524-4636     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-01-29     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2929-37     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / drug effects,  metabolism*
Biological Markers / metabolism
Cell Line
Dose-Response Relationship, Drug
Eicosapentaenoic Acid / pharmacology
Fatty Acids / pharmacology*
Fatty Acids, Omega-3 / pharmacology*
Interleukin-12 Subunit p40 / metabolism
Interleukin-6 / metabolism*
Lipase / metabolism*
Lipopolysaccharides / pharmacology
Macrophages, Peritoneal / drug effects,  metabolism*
Male
Mice
Mice, Knockout
Models, Animal
PPAR gamma / metabolism*
Palmitic Acid / pharmacology
Receptors, LDL / deficiency,  genetics,  metabolism
Toll-Like Receptor 4 / metabolism
Grant Support
ID/Acronym/Agency:
5K08AG025833/AG/NIA NIH HHS; HL 40404/HL/NHLBI NIH HHS; R01 HL040404/HL/NHLBI NIH HHS; T32 DK007647/DK/NIDDK NIH HHS; T32 HL007343/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fatty Acids; 0/Fatty Acids, Omega-3; 0/Interleukin-12 Subunit p40; 0/Interleukin-6; 0/Lipopolysaccharides; 0/PPAR gamma; 0/Receptors, LDL; 0/Tlr4 protein, mouse; 0/Toll-Like Receptor 4; 2V16EO95H1/Palmitic Acid; AAN7QOV9EA/Eicosapentaenoic Acid; EC 3.1.1.-/Lipg protein, mouse; EC 3.1.1.3/Lipase
Comments/Corrections

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