Document Detail


Fatty acid selectivity of lipases during acidolysis reaction between oleic acid and monoacid triacylglycerols.
MedLine Citation:
PMID:  19835376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With the aim of determining the fatty acid (FA) selectivity of lipases, a mixture of oleic acid and monoacid triacylglycerols (TAGs) including tricaproin (T6), tricaprylin (T8), tricaprin (T10), trilaurin (T12), trimyristin (T14), tripalmitin (T16) and tristearin (T18) was used as the substrate in acidolysis performed in hexane. Three immobilized lipases, namely, Lipozyme TL IM from Thermomyces lanoginosus, Lipozyme RM IM from Rhizomucor miehei and Novozym 435 from Candida antarctica, were used as biocatalyst. The effects of operating variables including the mole ratio of oleic acid to monoacid TAG, temperature, enzyme dosage and reaction time on incorporation were also investigated. Significantly different incorporation rates were obtained for different TAGs used (P < 0.05). Incorporation of oleic acid into TAGs except tricaproin and tricaprylin was higher for all the TAGs with Lipozyme TL IM catalyzed reactions than those of other two enzymes tested. Incorporation of oleic acid decreased as the acyl chain length of FA in the TAG increased with Novozyme 435 catalyzed acidolysis. Compared to the other substrate mixtures, the highest incorporation was observed for oleic acid and tricaproin mixture with three lipases tested. It was shown that the FA selectivity of the lipases is strongly dependent on the acyl chain length of FA in a TAG.
Authors:
Ihsan Karabulut; Gokhan Durmaz; Ali Adnan Hayaloglu
Related Documents :
24274756 - Evidence for right ventricular lipotoxicity in heritable pulmonary arterial hypertension.
3936376 - Influences of metal ions on the reaction of amino and imino acids with fluorogenic reag...
2864316 - Pharmacological characterization of mouse ear pca.
20823616 - Nitrosation of n-methyl derivatives of uric acid and their transnitrosation ability to ...
11525406 - Induction of jlba mrna synthesis for a putative bzip protein of aspergillus nidulans by...
74186 - Lysosomal (leucocyte) proteinase and sulfatase levels in dyggve-melchior-clausen (dmc) ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10466-70     Citation Subset:  IM    
Affiliation:
Department of Food Engineering, Inonu University, 44280 Malatya, Turkey. ikarabulut@inonu.edu.tr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Catalysis
Fatty Acids / chemistry*
Fungal Proteins / chemistry*
Fungi / chemistry,  enzymology*
Kinetics
Lipase / chemistry*
Oleic Acid / chemistry
Substrate Specificity
Triglycerides / chemistry
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fungal Proteins; 0/Triglycerides; 112-80-1/Oleic Acid; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Polymerization of Enantiopure Monomers Using Syndiospecific Catalysts: A New Approach To Sequence Co...
Next Document:  Identification of the Bioactive Constituent and Its Mechanisms of Action in Mediating the Anti-Infla...