Document Detail

Fatty-acid metabolism is involved in stress-resistance mechanisms of Caenorhabditis elegans.
MedLine Citation:
PMID:  19896458     Owner:  NLM     Status:  MEDLINE    
Fatty acids are the major components of the phospholipid bilayer and are involved in several functions of cell membrane. We previously reported that fatty-acid metabolism is involved in the regulation of DAF-2/insulin signal in Caenorhabditis elegans. In this study, we investigate the role of fatty-acid metabolism in stress resistance with respect to daf-16 in nematode. We found that fatty-acid metabolism regulates heat, osmotic, and oxidative-stress resistance in C. elegans. RNA interference (RNAi) of fat-6, fat-7, and elo-2 enhanced heat resistance but decreased oxidative-stress tolerance. RNAi of fat-2 strongly increased osmotic-stress resistance, whereas nhr-49-RNAi remarkably reduced osmotic and oxidative-stress tolerance. In daf-16 mutants (mgDf50), RNAi of fat-2 and fat-7 increased viability under osmotic stress, while RNAi of fat-6, fat-7, and elo-2 enhanced heat resistance. Exposure of saturated fatty acids to RNAi worms of fat-1-, fat-7-, and nhr-49 increased osmotic resistance. On the other hand, polyunsaturated fatty acids (PUFAs) reduced osmotic-stress tolerance in fat-2-RNAi worms, whereas PUFAs enhanced it in nhr-49-RNAi worms. Heat-stress resistance in fat-6- and fat-7-RNAi worms was suppressed by oleic acid. These results suggest that stress-resistance mechanisms are regulated by fatty-acid metabolism with or without DAF-16 activity.
Makoto Horikawa; Kazuichi Sakamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-05
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  390     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-30     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1402-7     Citation Subset:  IM    
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
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MeSH Terms
Caenorhabditis elegans / genetics,  metabolism,  physiology*
Caenorhabditis elegans Proteins / metabolism
Fatty Acids / genetics,  metabolism*
Heat-Shock Response*
Insulin / metabolism
Osmotic Pressure
Oxidative Stress*
RNA Interference
Receptor, Insulin / metabolism
Transcription Factors / metabolism
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/DAF-2 protein, C elegans; 0/Fatty Acids; 0/Transcription Factors; 0/daf-16 protein, C elegans; 11061-68-0/Insulin; EC, Insulin

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