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Fatigue biomarker index: An objective salivary measure of fatigue level.
MedLine Citation:
PMID:  22239935     Owner:  NLM     Status:  In-Data-Review    
Fatigue changed the composition of the small-molecular weight (sMW) proteome of saliva during a 10h session of moderate (70% of maximum ventilatory threshold) physical exertion. Saliva samples were collected from nine recreationally trained cyclists participating in a cross-over study designed to simulate prolonged manual labor, a military operation or wildfire-suppression work. During each hour of the study, participants performed an exercise program that included upper and lower body exercises separated by short periods of recovery. Over the course of the study, fatigue level increased as suggested by a significant increase in the participants' relative perceived exertion. The composition of the sMW proteome was investigated using reversed-phase liquid chromatography with mass-spectrometric detection. Isotopes of acetic anhydride were used for mass-specific labeling of samples and subsequent identification of ions with significant changes in intensity. Cluster analysis was used to identify a pair of peptides with concentrations that changed in opposite directions with fatigue level, i.e. concentration of one peptide increased while concentration of the other decreased. The sequences of the two peptides were determined by high-resolution mass spectrometry. The ratio of the ion intensities of these two peptides, referred to as the fatigue biomarker index, was calculated for subjects throughout the study. The FBI values from the start of the study likely arose from a different distribution than the FBI values measured at the end of the study (Mann-Whitney test, P<.05). While this study is restricted to a small population of recreationally trained cyclists performing exercise under controlled conditions, it holds promise for the development of an objective salivary measurement of fatigue that is applicable to a much broader population performing in uncontrolled environments.
Darren J Michael; Sheena Daugherty; Adrienne Santos; Brent C Ruby; John E Kalns
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Publication Detail:
Type:  Journal Article     Date:  2011-10-10
Journal Detail:
Title:  Accident; analysis and prevention     Volume:  45 Suppl     ISSN:  1879-2057     ISO Abbreviation:  Accid Anal Prev     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-01-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254476     Medline TA:  Accid Anal Prev     Country:  England    
Other Details:
Languages:  eng     Pagination:  68-73     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Hyperion Biotechnology, Inc., 13302 Langtry Rd., San Antonio, TX 78248, United States.
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