Document Detail

Fate of rubella genome ribonucleic acid after immune and nonimmune virolysis in the presence of ribonuclease.
MedLine Citation:
PMID:  992869     Owner:  NLM     Status:  MEDLINE    
To determine whether rubella virion ribonucleic acid (RNA) becomes accessible to nuclease attack after immune lysis of the viral envelope, virions containing radioactively labeled RNA were examined in three ways with the following results. (i) Incubation of purified virus with heat-inactivated rubella convalescent human serum and guinea pig complement resulted in an increase in acid-soluble RNA. Antibody was required; the reaction was temperature dependent and was blocked by ethylenediaminetetraacetic acid. When exogenous nuclease was added prior to lysis, radioactivity in virions was reduced to 15% of that in unlysed control pellets (ii) Sucrose gradient sedimentation profiles of RNA released from lysed and unlysed virions under controlled conditions showed that the nuclease content of serum-virus mixtures was sufficient to eliminate all RNA of genome size, although degradation was not complete. (iii) Virions were also lysed by unheated human immune sera in the absence of guinea pig complement and by some, but not all, unheated antibody-negative sera.
A Schluederberg; C Ajello; B Evans
Related Documents :
3301459 - Attempts for replacement of asparagine in sauton's medium by some organic acids in the ...
7300529 - Steep gradients of amino acids between cochlear endolymph and perilymph.
7390399 - Hydrazonopropionic acid, a new class of hypoglycemic substances. 2. influence of 2-(phe...
1452399 - Anticomplementary activity of boswellic acids--an inhibitor of c3-convertase of the cla...
647159 - Tachyphylaxis to ethacrynic acid in the isolated atrium of guinea-pig and its relation ...
7791089 - Pharmacodynamics of 15(s)-hydroperoxyeicosatetraenoic (15-hpete) and 15(s)-hydroxyeicos...
8638429 - Variability of vitamin e content in pumpkin seeds (cucurbita pepo l.).
1619039 - Role of arachidonic acid metabolism on corticotropin-releasing factor (crf)-release ind...
12147729 - Induced parasitoid attraction by arabidopsis thaliana: involvement of the octadecanoid ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  14     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1977-01-28     Completed Date:  1977-01-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1097-102     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acetic Acids / metabolism
Antibody Specificity
Centrifugation, Density Gradient
Cytotoxicity Tests, Immunologic
RNA, Viral / analysis*,  immunology,  metabolism
Ribonucleases / metabolism*
Rubella virus / analysis*,  immunology
Reg. No./Substance:
0/Acetic Acids; 0/RNA, Viral; 10028-17-8/Tritium; EC 3.1.-/Ribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Immunological control mechanism against cholera toxin: interference with toxin binding to intestinal...
Next Document:  Passive hemolysis test for antibody to Treponema hyodysenteriae.