Document Detail


Fate of rubella genome ribonucleic acid after immune and nonimmune virolysis in the presence of ribonuclease.
MedLine Citation:
PMID:  992869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine whether rubella virion ribonucleic acid (RNA) becomes accessible to nuclease attack after immune lysis of the viral envelope, virions containing radioactively labeled RNA were examined in three ways with the following results. (i) Incubation of purified virus with heat-inactivated rubella convalescent human serum and guinea pig complement resulted in an increase in acid-soluble RNA. Antibody was required; the reaction was temperature dependent and was blocked by ethylenediaminetetraacetic acid. When exogenous nuclease was added prior to lysis, radioactivity in virions was reduced to 15% of that in unlysed control pellets (ii) Sucrose gradient sedimentation profiles of RNA released from lysed and unlysed virions under controlled conditions showed that the nuclease content of serum-virus mixtures was sufficient to eliminate all RNA of genome size, although degradation was not complete. (iii) Virions were also lysed by unheated human immune sera in the absence of guinea pig complement and by some, but not all, unheated antibody-negative sera.
Authors:
A Schluederberg; C Ajello; B Evans
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  14     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1977-01-28     Completed Date:  1977-01-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1097-102     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetic Acids / metabolism
Antibody Specificity
Centrifugation, Density Gradient
Cytotoxicity Tests, Immunologic
Humans
RNA, Viral / analysis*,  immunology,  metabolism
Ribonucleases / metabolism*
Rubella virus / analysis*,  immunology
Solubility
Tritium
Chemical
Reg. No./Substance:
0/Acetic Acids; 0/RNA, Viral; 10028-17-8/Tritium; EC 3.1.-/Ribonucleases
Comments/Corrections

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