Document Detail

Fate of newly synthesized histones in G1 and G0 cells.
MedLine Citation:
PMID:  6617889     Owner:  NLM     Status:  MEDLINE    
We have shown that quiescent cells as well as those in the G1 phase of the cell cycle synthesize histones at a reduced but significant rate. Now, we show that the histones synthesized during G0 and G1 are stably incorporated into nuclei soon after synthesis. Micrococcal nuclease digestion of nuclei isolated from cells in G0 and G1 revealed that the specific histone variants synthesized in these different physiological states are found associated with DNA as nucleosomes. Nucleosomes were separated by polyacrylamide gel electrophoresis in a reducing buffer so that histone spot morphology, particularly that of the H3s was improved.
R S Wu; L J Perry; W M Bonner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  162     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  161-6     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Nucleus / metabolism
Electrophoresis, Polyacrylamide Gel
Histones / biosynthesis*
Nucleosomes / metabolism
Reg. No./Substance:
0/Histones; 0/Nucleosomes

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