| Fate of newly synthesized histones in G1 and G0 cells. | |
| | |
MedLine Citation:
|
PMID: 6617889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have shown that quiescent cells as well as those in the G1 phase of the cell cycle synthesize histones at a reduced but significant rate. Now, we show that the histones synthesized during G0 and G1 are stably incorporated into nuclei soon after synthesis. Micrococcal nuclease digestion of nuclei isolated from cells in G0 and G1 revealed that the specific histone variants synthesized in these different physiological states are found associated with DNA as nucleosomes. Nucleosomes were separated by polyacrylamide gel electrophoresis in a reducing buffer so that histone spot morphology, particularly that of the H3s was improved. |
| | |
Authors:
|
R S Wu; L J Perry; W M Bonner |
Related Documents
:
|
18077559 - Biphasic incorporation of centromeric histone cenp-a in fission yeast. 12406559 - Apoptosis sensitivity is not correlated with sensitivity to proliferation inhibition by... 20569199 - Genomic tagging reveals a random association of endogenous ptdins5p 4-kinases iialpha a... 10223179 - Colonic epithelial cell activation and the paradoxical effects of butyrate. 15258579 - B cell-specific loss of histone 3 lysine 9 methylation in the v(h) locus depends on pax5. 17612559 - The hdac inhibitor trichostatin a inhibits growth of small cell lung cancer cells. 1826759 - Anchoring and degradation of glycolipid-anchored membrane proteins by l929 versus by lm... 469479 - In vitro synthesis of rna by xenopus spermatogenic cells i. evidence for polyadenylated... 7853479 - Adeno-associated virus vectors transduce primary cells much less efficiently than immor... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: FEBS letters Volume: 162 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1983 Oct |
Date Detail:
|
Created Date: 1983-11-23 Completed Date: 1983-11-23 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 161-6 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Cell Nucleus / metabolism Cricetinae Electrophoresis, Polyacrylamide Gel Female Histones / biosynthesis* Interphase* Nucleosomes / metabolism Ovary |
| Chemical | |
Reg. No./Substance:
|
0/Histones; 0/Nucleosomes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reduced mitogenic stimulation of human lymphocytes by extremely low frequency electromagnetic fields...
Next Document: Synthesis of phage-specific transfer RNA molecules by vibriophage phi 149.