Document Detail


Fate and effect of ingested Bacillus cereus spores and vegetative cells in the intestinal tract of human-flora-associated rats.
MedLine Citation:
PMID:  16420599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fate and effect of Bacillus cereus F4433/73R in the intestine of human-flora-associated rats was studied using bacteriological culturing techniques and PCR-denaturing gradient gel electrophoresis in combination with cell assays and immunoassays for detection of enterotoxins. In faecal samples from animals receiving vegetative cells, only few B. cereus cells were detected. Spores survived the gastric barrier well, and were in some cases detected up to 2 weeks after ingestion. Selective growing revealed no major changes in the intestinal flora during passage of B. cereus. However, denaturing gradient gel electrophoresis analysis with universal 16S rRNA gene primers revealed significant changes in the intestinal microbiota of animals dosed with spores. Vero cell assays and a commercial kit (BCET-RPLA) did not reveal any enterotoxin production from B. cereus F4433/73R in the intestinal tract.
Authors:
Andrea Wilcks; Bjarne Munk Hansen; Niels Bohse Hendriksen; Tine Rask Licht
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  46     ISSN:  0928-8244     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-19     Completed Date:  2006-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  70-7     Citation Subset:  IM    
Affiliation:
Department of Microbiological Food Safety, Danish Institute for Food and Veterinary Research, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark. anw@dfvf.dk
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacillus cereus / genetics,  isolation & purification,  physiology*
Cercopithecus aethiops
DNA, Bacterial / analysis
Enterotoxins / analysis,  biosynthesis
Feces / microbiology
Humans
Intestines / microbiology*
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics
Rats
Rats, Sprague-Dawley
Spores / genetics,  isolation & purification,  physiology*
Vero Cells
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Enterotoxins; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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