Document Detail


The fate of ZnO nanoparticles administered to human bronchial epithelial cells.
MedLine Citation:
PMID:  22646753     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A particular challenge for nanotoxicology is the evaluation of the biological fate and toxicity of nanomaterials that dissolve in aqueous fluids. Zinc oxide nanomaterials are of particular concern because dissolution leads to release of the toxic divalent zinc ion. Although zinc ions have been implicated in ZnO cytotoxicity, direct identification of the chemical form of zinc taken up by cells exposed to ZnO nanoparticles, and its intracellular fate, has not yet been achieved. We combined high resolution X-ray spectromicroscopy and high elemental sensitivity X-ray microprobe analyses to determine the fate of ZnO and less soluble iron-doped ZnO nanoparticles following exposure to cultures of human bronchial epithelial cells, BEAS-2B. We complemented two-dimensional X-ray imaging methods with atomic force microscopy of cell surfaces to distinguish between nanoparticles that were transported inside the cells from those that adhered to the cell exterior. The data suggest cellular uptake of ZnO nanoparticles is a mechanism of zinc accumulation in cells. Following uptake, ZnO nanoparticles dissolved completely generating intracellular Zn(2+) complexed by molecular ligands. These results corroborate a model for ZnO nanoparticle toxicity that is based on nanoparticle uptake followed by intracellular dissolution.
Authors:
Benjamin Gilbert; Sirine C Fakra; Tian Xia; Suman Pokhrel; Lutz Mädler; André E Nel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-06-07
Journal Detail:
Title:  ACS nano     Volume:  6     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-26     Completed Date:  2012-11-07     Revised Date:  2014-07-15    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4921-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bronchi / chemistry,  drug effects*,  physiology*
Cell Line
Cell Survival / drug effects
Endocytosis / drug effects,  physiology*
Humans
Materials Testing
Nanoparticles / chemistry*,  toxicity*
Particle Size
Zinc Oxide / chemistry*,  toxicity*
Grant Support
ID/Acronym/Agency:
R01 ES016746/ES/NIEHS NIH HHS; R01 ES016746/ES/NIEHS NIH HHS; U19 ES019528/ES/NIEHS NIH HHS; U19 ES019528/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
SOI2LOH54Z/Zinc Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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