Document Detail


Fate specification and tissue-specific cell cycle control of the Caenorhabditis elegans intestine.
MedLine Citation:
PMID:  20053685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Coordination between cell fate specification and cell cycle control in multicellular organisms is essential to regulate cell numbers in tissues and organs during development, and its failure may lead to oncogenesis. In mammalian cells, as part of a general cell cycle checkpoint mechanism, the F-box protein beta-transducin repeat-containing protein (beta-TrCP) and the Skp1/Cul1/F-box complex control the periodic cell cycle fluctuations in abundance of the CDC25A and B phosphatases. Here, we find that the Caenorhabditis elegans beta-TrCP orthologue LIN-23 regulates a progressive decline of CDC-25.1 abundance over several embryonic cell cycles and specifies cell number of one tissue, the embryonic intestine. The negative regulation of CDC-25.1 abundance by LIN-23 may be developmentally controlled because CDC-25.1 accumulates over time within the developing germline, where LIN-23 is also present. Concurrent with the destabilization of CDC-25.1, LIN-23 displays a spatially dynamic behavior in the embryo, periodically entering a nuclear compartment where CDC-25.1 is abundant.
Authors:
Alexandra Segref; Juan Cabello; Caroline Clucas; Ralf Schnabel; Iain L Johnstone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-06
Journal Detail:
Title:  Molecular biology of the cell     Volume:  21     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-26     Completed Date:  2010-07-26     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  725-38     Citation Subset:  IM    
Affiliation:
Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / metabolism
Cell Cycle
Cell Cycle Proteins / metabolism
Cell Lineage
Cell Nucleus / metabolism
F-Box Proteins / metabolism
Gene Expression Regulation, Developmental*
Immunohistochemistry / methods
Intestines / cytology*
Microscopy, Confocal / methods
Models, Biological
Models, Genetic
Phenotype
RNA Interference
cdc25 Phosphatases / metabolism
Grant Support
ID/Acronym/Agency:
//Medical Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Cell Cycle Proteins; 0/F-Box Proteins; 0/lin-23 protein, C elegans; EC 3.1.3.48/cdc25 Phosphatases
Comments/Corrections

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