Document Detail


Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes.
MedLine Citation:
PMID:  11158926     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regional differences in free fatty acid (FFA) handling contribute to diseases associated with particular fat distributions. As cultured rat preadipocytes became differentiated, FFA transfer into preadipocytes increased and was more rapid in single perirenal than in epididymal cells matched for lipid content. Uptake by human omental preadipocytes was greater than uptake by abdominal subcutaneous preadipocytes. Adipose-specific fatty acid binding protein (aP2) and keratinocyte lipid binding protein abundance was higher in differentiated rat perirenal than in epididymal preadipocytes. This interdepot difference in preadipocyte aP2 expression was reflected in fat tissue in older animals. Carnitine palmitoyltransferase 1 activity increased during differentiation and was higher in perirenal than in epididymal preadipocytes, particularly the muscle isoform. Long-chain acyl-CoA levels were higher in perirenal than in epididymal preadipocytes and isolated fat cells. These data are consistent with interdepot differences in fatty acid flux ensuing from differences in fatty acid binding proteins and enzymes of fat metabolism. Heterogeneity among depots results, in part, from distinct intrinsic characteristics of adipose cells. Different depots are effectively separate miniorgans.
Authors:
F Caserta; T Tchkonia; V N Civelek; M Prentki; N F Brown; J D McGarry; R A Forse; B E Corkey; J A Hamilton; J L Kirkland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  280     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E238-47     Citation Subset:  IM    
Affiliation:
Obesity Research Center, Evans Department of Medicine, Boston University Medical Center, Boston, Massachusetts 02118, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / metabolism
Adipocytes / metabolism*
Adult
Animals
Carnitine O-Palmitoyltransferase / metabolism
Carrier Proteins / metabolism
Cells, Cultured
Epididymis
Fatty Acid-Binding Proteins
Fatty Acids, Nonesterified / metabolism*
Female
Humans
Kidney
Male
Middle Aged
Neoplasm Proteins*
Nerve Tissue Proteins*
Omentum / cytology
Rats
Rats, Inbred F344
Stem Cells / metabolism*
Substrate Specificity
Tumor Suppressor Proteins*
Grant Support
ID/Acronym/Agency:
AG/DK-13925/AG/NIA NIH HHS; DK-18573/DK/NIDDK NIH HHS; DK-46200/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Carrier Proteins; 0/FABP7 protein, human; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids, Nonesterified; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Tumor Suppressor Proteins; EC 2.3.1.21/Carnitine O-Palmitoyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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