| Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes. | |
| | |
MedLine Citation:
|
PMID: 11158926 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Regional differences in free fatty acid (FFA) handling contribute to diseases associated with particular fat distributions. As cultured rat preadipocytes became differentiated, FFA transfer into preadipocytes increased and was more rapid in single perirenal than in epididymal cells matched for lipid content. Uptake by human omental preadipocytes was greater than uptake by abdominal subcutaneous preadipocytes. Adipose-specific fatty acid binding protein (aP2) and keratinocyte lipid binding protein abundance was higher in differentiated rat perirenal than in epididymal preadipocytes. This interdepot difference in preadipocyte aP2 expression was reflected in fat tissue in older animals. Carnitine palmitoyltransferase 1 activity increased during differentiation and was higher in perirenal than in epididymal preadipocytes, particularly the muscle isoform. Long-chain acyl-CoA levels were higher in perirenal than in epididymal preadipocytes and isolated fat cells. These data are consistent with interdepot differences in fatty acid flux ensuing from differences in fatty acid binding proteins and enzymes of fat metabolism. Heterogeneity among depots results, in part, from distinct intrinsic characteristics of adipose cells. Different depots are effectively separate miniorgans. |
| | |
Authors:
|
F Caserta; T Tchkonia; V N Civelek; M Prentki; N F Brown; J D McGarry; R A Forse; B E Corkey; J A Hamilton; J L Kirkland |
Related Documents
:
|
1588836 - Organic acid profiling in adipocyte differentiation of 3t3-f442a cells: increased produ... 15952896 - Monitoring energy balance: metabolites of fatty acid synthesis as hypothalamic sensors. 567166 - Fatty acid synthesis in mice during the 24hr cycle and during meal-feeding. 15372196 - Regeneration of pea (pisum sativum l.) by a cyclic organogenic system. 1588836 - Organic acid profiling in adipocyte differentiation of 3t3-f442a cells: increased produ... 961446 - Gastric clearance of gastrin during acid secretory responses to vagal activation and it... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: American journal of physiology. Endocrinology and metabolism Volume: 280 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2001 Feb |
Date Detail:
|
Created Date: 2001-02-22 Completed Date: 2001-03-29 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
|
Languages: eng Pagination: E238-47 Citation Subset: IM |
Affiliation:
|
Obesity Research Center, Evans Department of Medicine, Boston University Medical Center, Boston, Massachusetts 02118, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acyl Coenzyme A
/
metabolism Adipocytes / metabolism* Adult Animals Carnitine O-Palmitoyltransferase / metabolism Carrier Proteins / metabolism Cells, Cultured Epididymis Fatty Acid-Binding Proteins Fatty Acids, Nonesterified / metabolism* Female Humans Kidney Male Middle Aged Neoplasm Proteins* Nerve Tissue Proteins* Omentum / cytology Rats Rats, Inbred F344 Stem Cells / metabolism* Substrate Specificity Tumor Suppressor Proteins* |
| Grant Support | |
ID/Acronym/Agency:
|
AG/DK-13925/AG/NIA NIH HHS; DK-18573/DK/NIDDK NIH HHS; DK-46200/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Acyl Coenzyme A; 0/Carrier Proteins; 0/FABP7 protein, human; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids, Nonesterified; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Tumor Suppressor Proteins; EC 2.3.1.21/Carnitine O-Palmitoyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DAG accumulation from saturated fatty acids desensitizes insulin stimulation of glucose uptake in mu...
Next Document: Uric acid inhibits liver phosphorylase a activity under simulated in vivo conditions.