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Fat accumulation in Caenorhabditis elegans is mediated by SREBP homolog SBP-1.
MedLine Citation:
PMID:  19936816     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Research into the metabolism of fats may reveal potential targets for developing pharmaceutical approaches to obesity and related disorders. Such research may be limited, however, by the cost and time involved in using mammalian subjects or developing suitable cell lines. To determine whether invertebrates could be used to carry out such research more efficiently, we investigated the ability of Caenorhabditis elegans (C. elegans) to accumulate body fat following the consumption of excess calories and the mechanisms it uses to metabolize fat. C. elegans worms were grown on media containing various sugars and monitored for changes in body fat and expression of sbp-1, a homolog of the mammalian transcription factor SREBP-1c, which facilitates fat storage in mammals. The fat content increased markedly in worms exposed to glucose. In situ analysis of gene expression in transgenic worms carrying the GFP-labeled promoter region of sbp-1 revealed that sbp-1 mRNA was strongly expressed in the intestine. An sbp-1 knockdown caused a reduction in body size, fat storage, and egg-laying activity. RT-PCR analysis revealed a considerable decrease in the expression of fatty acid synthetic genes (including elo-2, fat-2, and fat-5) and a considerable increase of starvation-inducible gene acs-2. Normal egg-laying activity and acs-2 expression were restored on exposure to a polyunsaturated fatty acid. These findings suggest that SBP-1 and SREBP regulate the amount and composition of fat and response to starvation in a similar manner. Thus, C. elegans may be an appropriate subject for studying the metabolism of fats.
Toshihisa Nomura; Makoto Horikawa; Satoru Shimamura; Teppei Hashimoto; Kazuichi Sakamoto
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Publication Detail:
Type:  Journal Article     Date:  2009-11-20
Journal Detail:
Title:  Genes & nutrition     Volume:  5     ISSN:  1555-8932     ISO Abbreviation:  Genes Nutr     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-06-17     Completed Date:  2011-07-14     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101280108     Medline TA:  Genes Nutr     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  17-27     Citation Subset:  -    
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki Japan.
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