Document Detail

Fasting whole blood as a biomarker of essential fatty acid intake in epidemiologic studies: comparison with adipose tissue and plasma.
MedLine Citation:
PMID:  16014782     Owner:  NLM     Status:  MEDLINE    
Biomarkers could provide a more accurate measure of long-term intake than questionnaires. Adipose tissue is considered the best indicator of long-term essential fatty acid intake, but other tissues may prove equally valid. The authors evaluated the ability of fasting whole blood, relative to fasting plasma and adipose tissue, to reflect fatty acid intake. Costa Rican men (n = 99) and women (n = 101) completed a 135-item food frequency questionnaire and provided adipose tissue and blood samples from 1999 to 2001. Fatty acids were identified by using capillary gas chromatography. Correlation coefficients adjusted for age, sex, and body mass index were calculated. Diet-tissue correlation coefficients for alpha-linolenic acid and linoleic acid, respectively, were 0.38 and 0.43 in whole blood, 0.51 and 0.52 in adipose tissue, and 0.39 and 0.41 in plasma. High correlations were observed between whole-blood alpha-linolenic and linoleic acid and adipose tissue (r = 0.59 and r = 0.67) and plasma (r = 0.96 and r = 0.88), respectively. Results show that fasting whole blood is a suitable biomarker of long-term essential fatty acid intake, and its performance is comparable to that of fasting plasma. Thus, fasting whole blood could be the sample of choice in epidemiologic studies because of its ability to predict intake, its accessibility, and minimum sample processing.
Ana Baylin; Mi Kyung Kim; Amy Donovan-Palmer; Xinia Siles; Lauren Dougherty; Paula Tocco; Hannia Campos
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-07-13
Journal Detail:
Title:  American journal of epidemiology     Volume:  162     ISSN:  0002-9262     ISO Abbreviation:  Am. J. Epidemiol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-03     Completed Date:  2005-08-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7910653     Medline TA:  Am J Epidemiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  373-81     Citation Subset:  IM    
Department of Nutrition, Harvard School of Public Health, Boston, MA 02115, USA.
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MeSH Terms
Adipose Tissue / chemistry*
Biological Markers / blood,  metabolism
Chromatography, Gas
Costa Rica
Diet Records
Dietary Fats / administration & dosage,  metabolism*
Fatty Acids, Essential / administration & dosage,  blood,  metabolism*
Middle Aged
Grant Support
Reg. No./Substance:
0/Biological Markers; 0/Dietary Fats; 0/Fatty Acids, Essential

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