| Fasting and postprandial apolipoprotein B-48 levels in healthy, obese, and hyperlipidemic subjects. | |
| | |
MedLine Citation:
|
PMID: 19592048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Apolipoprotein (apo) B-48 is the only specific marker of intestinal lipoproteins. We evaluated a novel enzyme-linked immunosorbent assay (ELISA) standardized with recombinant apo B-48 to measure apo B-48 in plasma and triglyceride-rich lipoproteins (TRLs, density <1.006 g/mL). Coefficients of variation were less than 2.5%. Assay values correlated well (r = 0.82, P < .001) with values obtained by gel scanning of TRLs (n = 75 samples); however, the gel scanning method yielded values that were about 50% lower than ELISA values. About 60% to 70% of apo B-48 was found in TRLs. In 12 healthy subjects, median fasting plasma apo B-48 levels were 0.51 mg/dL and were increased by 121% to 147% in the fed state. In 63 obese subjects, median fasting apo B-48 values were 0.82 mg/dL; and feeding resulted in almost no change in total cholesterol, non-high-density lipoprotein cholesterol, or total apo B values, whereas triglyceride, remnant lipoprotein cholesterol, and apo B-48 levels were significantly higher (P < .05; by +73%, +58%, and +106%), and direct low-density lipoprotein cholesterol and direct high-density lipoprotein cholesterol were significantly lower (P < .001, by -13% and -20%) than fasting values. Relative to controls, 270 hyperlipidemic subjects had significantly higher (P < .001, +115%) fasting total apo B and higher apo B-48 values (P = .06, +37%). Our data indicate that the apo B-48 ELISA tested provides highly reproducible results and is excellent for research studies. Median apo B-48 values in healthy subjects are about 0.5 mg/dL and increase more than 100% in the fed state. Elevated levels are observed in obese and hyperlipidemic subjects. |
| | |
Authors:
|
Seiko Otokozawa; Masumi Ai; Margaret R Diffenderfer; Bela F Asztalos; Akira Tanaka; Stefania Lamon-Fava; Ernst J Schaefer |
Related Documents
:
|
6787158 - Rat plasma lipoproteins and apolipoproteins in experimental hypothyroidism. 6435608 - Intestinal biosynthesis of apolipoproteins during active fat absorption in rabbits. 7706948 - Identification and characterization of a novel apolipoprotein e variant, apolipoprotein... 2767788 - Lipoprotein and apoprotein levels in different types of dialysis. 2607528 - The influence of breed and sex on the incidence of mortalities and skin tears in broile... 21811018 - Processing of endogenous aβpp in blood-brain barrier endothelial cells is modulated by... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-07-09 |
Journal Detail:
|
Title: Metabolism: clinical and experimental Volume: 58 ISSN: 1532-8600 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-10-19 Completed Date: 2009-11-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0375267 Medline TA: Metabolism Country: United States |
Other Details:
|
Languages: eng Pagination: 1536-42 Citation Subset: IM |
Affiliation:
|
Lipid Metabolism Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apolipoprotein B-48
/
blood* Body Mass Index Cholesterol / blood Cholesterol, HDL / blood Cholesterol, LDL / blood Enzyme-Linked Immunosorbent Assay Fasting / physiology* Female Humans Hyperlipidemias / blood* Lipoproteins / blood Male Middle Aged Obesity / blood* Postprandial Period / physiology* Reference Values Triglycerides / blood |
| Grant Support | |
ID/Acronym/Agency:
|
HL74753/HL/NHLBI NIH HHS; P050HL083813/HL/NHLBI NIH HHS; R01 HL-60935/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Apolipoprotein B-48; 0/Cholesterol, HDL; 0/Cholesterol, LDL; 0/Lipoproteins; 0/Triglycerides; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Diagnostic lessons of recurrence pattern after transient ischemic attacks.
Next Document: The influence of sex, body composition, and nonesterified fatty acids on serum adipokine concentrati...