Document Detail


Fast separation of triterpenoids by supercritical fluid chromatography/evaporative light scattering detector.
MedLine Citation:
PMID:  23141985     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The screening of plant material, the chemical composition, the abundance and the biological activity of triterpenoids are of a major economical importance. The classical analytical methods, such as TLC, GC, and HPLC are either little resolutive, or require derivatization steps, or fail in sensitivity. The supercritical fluid chromatography/evaporative light scattering detector (SFC/ELSD) coupling provides high resolution, fast analysis and higher responses for the analysis of triterpenoids. After the initial screening of seven stationary phases to select the well suited one, analytical conditions (modifier percentage, from 10 to 3%; backpressure (from 12 to 18MPa) and temperature (from 15 to 25°C) were studied to improve the separation, and ELSD detection of a standard mixture composed of 8 triterpenoids (oleanolic acid, erythrodiol, β-amyrin, ursolic acid, uvaol, betulinic acid, betulin, lupeol). Applied to apple pomace extracts, this method allows the separation of about 15 triterpenoid compounds, in less than 20min, with isocratic conditions. Moreover, the ELSD response is dramatically higher than the one provided by UV detection, and avoids derivatization steps. An attempt to identify some compounds was done by collecting chromatographic peaks and further analyzing them with mass spectrometry. Complete identification or molecular formula could be proposed for 11 compounds. However, due to the presence of position and orientation isomers the absolute identification remains difficult, despite some retention rules deduced from the standard analysis.
Authors:
E Lesellier; E Destandau; C Grigoras; L Fougère; C Elfakir
Related Documents :
7881535 - High-precision gas chromatography-combustion isotope ratio mass spectrometry at low sig...
24645505 - Certification of elements in and use of standard reference material 3280 multivitamin/m...
18293285 - Pretreatment technique for siderite removal for organic carbon isotope and c:n ratio an...
14755615 - Continuous-flow isotope analysis of the deuterium/hydrogen ratio in atmospheric hydrogen.
20589845 - Complete anaerobic mineralization of pentachlorophenol (pcp) under continuous flow cond...
6587405 - Preparation of prostaglandin e2 vaginal gel for preinduction ripening of the cervix.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-22
Journal Detail:
Title:  Journal of chromatography. A     Volume:  -     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans, CNRS UMR 7311, B.P. 6759, rue de Chartres, 45067 Orléans Cedex 2, France. Electronic address: eric.lesellier@univ-orleans.fr.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Is entire removal of a post-traumatic temporomandibular joint ankylotic site necessary for an optima...
Next Document:  Recent developments on polysaccharide-based chiral stationary phases for liquid-phase separation of ...