| Fast rewarming after deep hypothermic circulatory arrest in rats impairs histologic outcome and increases NFκB expression in the brain. | |
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MedLine Citation:
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PMID: 20647245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Deep hypothermia is used as a neuroprotectant during cardiac surgery utilizing deep hypothermic circulatory arrest (DHCA), although the ideal rewarming strategy is not known yet. Some of the neuroprotective properties of hypothermia seem to be mediated by Nuclear Factor Kappa B (NFκB) as an important transcription factor. The current study was designed to investigate the effect of the rewarming rate on histologic outcome and cerebral NFκB expression one day following DHCA in rats. METHODS: With IRB approval, 20 rats were cannulated for cardiopulmonary bypass (CPB), cooled to a rectal temperature of 15-18°C, subjected to 45min of DHCA and randomly assigned to either a slow (40 min) or a fast (20 min) rewarming protocol. At 24 hours post DHCA, the number of eosinophilic neurons was analyzed with hematoxylin and eosin (HE) staining, and NFκB expression immunohistochemically. The two experimental groups were compared with untreated control rats. RESULTS: HE staining showed more eosinophilic neurons in the motor cortex following fast rewarming (60 [15-388]) compared to slow rewarming (15 [10-21]) (p<0.05). Neuronal expression of NFκB was increased in the fast rewarming group in both brain areas, the motor cortex (fast: 258 [135-393]; slow: 165 [80-212]; control: 73 [44-111]) as well as the hippocampus (fast: 243 [209-314]; slow: 202 [187-239]; control: 86 [68-108]) (p<0.05). Hyperthermic episodes were strictly avoided. CONCLUSIONS: Fast rewarming with strict avoidance of hyperthermia after DHCA in rats was accompanied by pronounced histologic damage and accentuated cerebral NFκB expression. |
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Authors:
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M Lucia Gordan; Kristine Kellermann; Manfred Blobner; Georg Nollert; Eberhard F Kochs; Bettina Jungwirth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-20 |
Journal Detail:
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Title: Perfusion Volume: 25 ISSN: 1477-111X ISO Abbreviation: Perfusion Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-23 Completed Date: 2011-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8700166 Medline TA: Perfusion Country: England |
Other Details:
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Languages: eng Pagination: 349-54 Citation Subset: IM |
Affiliation:
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Klinik für Anaesthesiologie, Technische Universität München, Klinikum rechts der Isar, Munich,Germany. l.gordan@lrz.tum.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry* Cardiopulmonary Bypass / methods* Cerebral Cortex Circulatory Arrest, Deep Hypothermia Induced / methods* Hippocampus NF-kappa B / biosynthesis* Rats Rewarming / methods* Time Factors Treatment Outcome |
| Chemical | |
Reg. No./Substance:
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0/NF-kappa B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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