Document Detail


Fast release of lipophilic agents from circulating PEG-PDLLA micelles revealed by in vivo forster resonance energy transfer imaging.
MedLine Citation:
PMID:  18257595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Understanding the in vivo behavior of nanoparticles is critical for the translation of nanomedicine from laboratory research to clinical trials. In this work, in vivo Forster resonance energy transfer (FRET) imaging was employed to monitor the release of hydrophobic molecules from circulating poly(ethylene glycol)-poly( D, L-lactic acid) (PEG-PDLLA) micelles. A lipophilic FRET pair (DiIC(18) and DiOC(18)) was physically entrapped into micelle cores by mimicking the loading of hydrophobic drugs. The FRET efficiency was found significantly reduced within 15 min after intravenous injection, implying that DiIC(18) and DiOC(18) quickly escaped from the circulating micelles. FRET spectroscopy studies further demonstrated that alpha- and beta-globulins were major factors for the observed fast release, while gamma-globulins, albumin, and red blood cells played minor roles. These results provide useful information for developing blood-stable micelles to deliver hydrophobic drugs to the target site via prolonged circulation and extravasation from the vascular system.
Authors:
Hongtao Chen; Sungwon Kim; Wei He; Haifeng Wang; Philip S Low; Kinam Park; Ji-Xin Cheng
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-02-08
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  24     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-13     Completed Date:  2008-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5213-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Weldon School of Biomedical Engineering, and Oncological Sciences Center, Purdue University, West Lafayette, Indiana 47907, USA.
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MeSH Terms
Descriptor/Qualifier:
Alpha-Globulins / chemistry
Animals
Beta-Globulins / chemistry
Biophysics / methods
Cattle
Drug Carriers / chemistry
Erythrocytes / metabolism
Fluorescence Resonance Energy Transfer / methods*
Mice
Mice, Inbred BALB C
Micelles*
Nanotechnology
Polyesters / chemistry*
Polyethylene Glycols / chemistry*
Polymers / chemistry
Serum Albumin / chemistry
Spectrometry, Fluorescence
Grant Support
ID/Acronym/Agency:
GM065284/GM/NIGMS NIH HHS; HL078715/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Alpha-Globulins; 0/Beta-Globulins; 0/Drug Carriers; 0/Micelles; 0/Polyesters; 0/Polyethylene Glycols; 0/Polymers; 0/Serum Albumin; 0/polyethylene glycol-polylactide-polyethylene glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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