Document Detail


Fast real-time PCR for the detection of crustacean allergen in foods.
MedLine Citation:
PMID:  22339490     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Crustaceans are one of the most common allergens causing severe food reaction. These food allergens are a health problem, and they have become very important; there are various regulations that establish that labeling must be present regarding these allergens to warn consumers. In the present work a fast real-time PCR, by a LNA probe, was developed. This allows the detection of crustaceans in all kinds of products, including processed products in which very aggressive treatments of temperature and pressure during the manufacturing process are used. This methodology provides greater sensitivity and specificity and reduces the analysis time of real-time PCR to 40 min. This methodology was further validated by means of simulating products likely to contain this allergen. For this, products present on the market were spiked with crustacean cooking water. The assay is a potential tool in issues related to the labeling of products and food security to protect the allergic consumer.
Authors:
Beatriz Herrero; Juan M Vieites; Montserrat Espiñeira
Publication Detail:
Type:  Journal Article     Date:  2012-02-16
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  60     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1893-7     Citation Subset:  IM    
Affiliation:
Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA , Vigo, 36310 Pontevedra, Spain.
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