| Fast ion-exchange HPLC of proteins using porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths grafted with poly(2-acrylamido-2-methyl-1-propanesulfonic acid). | |
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MedLine Citation:
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PMID: 9336979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths with different porous properties grafted with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) chains using cerium(IV) initiated free-radical polymerization have been prepared and used for the separation of proteins in ion-exchange HPLC mode. Because of the presence of the large pores that are typical of monolithic separation media which allow easy flow of all of the mobile phase, the efficiency of the columns does not deteriorate even at high flow velocities as a result of the specific morphology of the monoliths. Optimization of the chromatographic conditions such as the shape of the mobile phase gradient and the flow rate allows for very fast separation of three proteins in less than 1.5 min. |
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Authors:
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C Viklund; F Svec; J M Fréchet; K Irgum |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biotechnology progress Volume: 13 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 1997 Sep-Oct |
Date Detail:
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Created Date: 1997-11-21 Completed Date: 1997-11-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 597-600 Citation Subset: B |
Affiliation:
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Department of Chemistry, University of California, Berkeley 94720-1460, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Chromatography, High Pressure Liquid / methods* Chymotrypsinogen / isolation & purification Indicators and Reagents Methylmethacrylates* Muramidase / isolation & purification Myoglobin / isolation & purification Permeability Polymers* Proteins / isolation & purification* Sulfonic Acids* |
| Grant Support | |
ID/Acronym/Agency:
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GM-48364-05/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Methylmethacrylates; 0/Myoglobin; 0/Polymers; 0/Proteins; 0/Sulfonic Acids; 27119-07-9/poly(2-acrylamido-2-methyl-1-propanesulfonic acid); 31743-77-8/GMA-EDMA; 9035-75-0/Chymotrypsinogen; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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