Document Detail


Fast functional assay of protein C in whole plasma using a snake venom activator: evaluation in patients with congenital and acquired protein C deficiencies.
MedLine Citation:
PMID:  3416483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both anticoagulant and amidolytic activities of protein C (PC) were measured using a snake venom activator in 4 patients with hereditary PC deficiency, 37 with disseminated intravascular coagulation (DIC), and 30 under stabilized warfarin therapy. The results were compared with those obtained by an immunological assay (ELISA). PC levels measured by different functional and immunological assays were very close in patients with hereditary PC deficiency and DIC. In patients under stable oral anticoagulant therapy, there was no detectable difference between amidolytic activity and antigen levels of PC in each patient plasma, whereas a decrease in anticoagulant activity was much more pronounced. These results indicate that the present activity assays measure PC specifically, and that the snake venom activator is capable of activating both carboxylated and hypocarboxylated forms of PC, but only anticoagulant assay can evaluate the physiological PC function in vitamin K-deficient states.
Authors:
H Takahashi; M Hanano; W Tatewaki; A Shibata
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  175     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-10-27     Completed Date:  1988-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  217-25     Citation Subset:  IM    
Affiliation:
First Department of Internal Medicine, Niigata University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Biological Assay
Blood Coagulation / drug effects
Chromogenic Compounds
Disseminated Intravascular Coagulation / blood
Enzyme-Linked Immunosorbent Assay
Humans
Protein C / analysis*,  metabolism
Protein C Deficiency
Snake Venoms / pharmacology
Warfarin / therapeutic use
Chemical
Reg. No./Substance:
0/Chromogenic Compounds; 0/Protein C; 0/Snake Venoms; 81-81-2/Warfarin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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