| Fast enzymatic saccharification of switchgrass after pretreatment with ionic liquids. | |
| | |
MedLine Citation:
|
PMID: 19918908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The pretreatment of cellulose using ionic liquids (ILs) has been shown to be an effective method for improving the enzymatic hydrolysis of cellulose; this technique affords a fast and complete saccharification of cellulose into reducing sugars (Dadi et al., Biotechnol Bioeng. 2006; 95:904-910; Liu and Chen, Chinese Sci Bull. 2006; 51:2432-2436; Zhao et al., J Biotechnol. 2009; 139:47-54). Motivated by these advances, this study examines the effect of IL-pretreatment on the enzymatic hydrolysis of purified xylan (as a model system of hemicellulose) and switchgrass (as a real lignocellulose). The IL-pretreatment resulted in no improvement in the hydrolysis of xylan. The likely reason is that pure xylan has a low degree of polymerization (DP), and is readily biodegraded even without any pretreatment. However, in real cellulosic materials (such as switchgrass), xylan is entrapped within the cellulosic matrix, and cannot be conveniently accessed by enzymes. Our data demonstrate that the IL-pretreatment of switchgrass significantly improved the enzymatic saccharification of both cellulose (96% D-glucose yield in 24 h) and xylan (63% D-xylose yield in 24 h). The compositional analysis of switchgrass suggests a lower lignin content after IL-pretreatment. In addition, the infrared spectrum of regenerated switchgrass indicates a lower substrate crystallinity, whereas the enzyme adsorption isotherm further implies that the regenerated substrate is more accessible to enzymes. This study has further confirmed that IL-pretreatment is an effective tool in enhancing the enzymatic hydrolysis of cellulosic biomass, and allowing a more complete saccharification. |
| | |
Authors:
|
Hua Zhao; Gary A Baker; Janet V Cowins |
Related Documents
:
|
20473548 - Enhanced expression of a recombinant bacterial laccase at low temperature and microaero... 8368028 - Physiological studies on xylanase production by penicillium funiculosum on some agricul... 14504838 - Purification and characterization of laccase from the white-rot fungus daedalea quercin... 2039228 - Fermentation and aerobic metabolism of cellodextrins by yeasts. 223718 - Distribution of pns myelin proteins and membrane enzymes in fractions isolated by conti... 18718818 - Immobilization of glucose oxidase on carbon paper electrodes modified with conducting p... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biotechnology progress Volume: 26 ISSN: 1520-6033 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2010 Jan-Feb |
Date Detail:
|
Created Date: 2010-02-15 Completed Date: 2010-05-24 Revised Date: 2011-05-26 |
Medline Journal Info:
|
Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
|
Languages: eng Pagination: 127-33 Citation Subset: IM |
Affiliation:
|
Chemistry Program, Savannah State University, Savannah, GA 31404, USA. huazhao98@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biomass Cellulase / chemistry, metabolism* Cellulose / chemistry Endo-1,4-beta Xylanases / chemistry, metabolism* Hydrolysis Ionic Liquids / chemistry* Lignin / chemistry Poaceae / chemistry* Solubility Trichoderma / enzymology Xylans / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Ionic Liquids; 0/Xylans; 9004-34-6/Cellulose; 9005-53-2/Lignin; EC 3.2.1.4/Cellulase; EC 3.2.1.8/Endo-1,4-beta Xylanases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Motion correction using an enhanced floating navigator and GRAPPA operations.
Next Document: Magnetic field homogenization of the human prefrontal cortex with a set of localized electrical coil...