Document Detail


Fast determination of antibiotics in whole blood.
MedLine Citation:
PMID:  23190049     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
There is a need for analytical methods capable of monitoring blood antibiotic levels in real time. Here we present a method for quantifying antibiotic levels in whole blood that does not require any sample pretreatment. The tests employ the enzyme penicillinase to assay for penicillin G, penicillin V and ampicillin using a flow-injected biosensor, the Enzyme Thermistor. Optimal flow rates, sample volumes and pH were determined to be 0.5 mL/min, 100 μL and 7.0, respectively. Analysis of the antibiotics diluted in buffer gave a linear range of 0.17-5.0 mM. Calibration curves prepared using blood spiked with the antibiotics gave a linear range of 0.17-2.0 mM. Linear regression values for all of the calibration curves were 0.998 or higher. Assay cycle time was 5 min. The relative standard deviation value for 100 determinations of a mock blood sample spiked with penicillin G was 6.71%. Despite the elimination of sample pretreatment, no detectable clogging or signal drift was observed. The assay provides a fast, simple, reliable analytical method for determining antibiotic concentrations in blood without the need for any sample pretreatment. This is an important first step towards developing a device capable of real-time monitoring of antibiotic levels in whole blood. The technology has the potential to significantly improve the outcomes of patients undergoing critical care.
Authors:
Q Chen; A Andersson; M Mecklenburg; B Xie
Related Documents :
1462989 - The determination of hemodialysis blood recirculation using blood urea nitrogen measure...
18810229 - Physiologic variability of vascular access blood flow for hemodialysis.
2707909 - Urea kinetic modelling by partial dialysate collection.
23489489 - Theory and practice of a variable dome splitter for gas chromatography-olfactometry.
24041739 - When all else fails: 21st century amateur radio as an emergency communications medium.
991469 - Blood access in hemodialysis.
12577139 - Arterial wall shear stress: observations from the bench to the bedside.
8094349 - Evaluation of calcium-antagonist (nifedipine) in the treatment of pre-eclampsia.
16026289 - Biochemical markers of cardiovascular damage from tobacco smoke.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-19
Journal Detail:
Title:  Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases     Volume:  -     ISSN:  1469-0691     ISO Abbreviation:  Clin. Microbiol. Infect.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9516420     Medline TA:  Clin Microbiol Infect     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases.
Affiliation:
Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Medical School of Xi'an Jiaotong University, Xi'an, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Dissociable contributions of the ventral hippocampus and orbitofrontal cortex to decision-making wit...
Next Document:  Differential regulation of collagen, lysyl oxidase and MMP-2 in human periodontal ligament cells by ...