| Fast detection of choline-containing metabolites in liver using 2D (1)H-(14)N three-bond correlation (HN3BC) spectroscopy. | |
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MedLine Citation:
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PMID: 22204824 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Detection and quantification of total choline-containing metabolites (CCMs) in tissues by magnetic resonance spectroscopy (MRS) has received considerable attention as a biomarker of cancer. Tissue CCMs are mainly choline (Cho), phosphocholine (PCho), and glycerophosphocholine (GPCho). Because the methyl (1)H resonances of tissue CCMs exhibit small chemical shift differences and overlap significantly in 1D (1)H MRS, quantification of individual components is precluded. Development of a MRS method capably of resolving individual components of tissue CCMs would be a significant advance. Herein, a modification of the 2D (1)H-(14)N HSQC technique is targeted on the two methylene (1)H in the CH(2)O group ((3)J(1H14N)=2.7Hz) and applied to ex vivo mouse and human liver samples at physiological temperature (37°C). Specifically, the (1)H-(14)N HSQC technique is modified into a 2D (1)H-(14)N three-bond correlation (HN3BC) experiment, which selectively detects the (1)H of CH(2)O coupled to (14)N in CCMs. Separate signals from Cho, PCho, and GPCho components are resolved with high detection sensitivity. A 2D HN3BC spectrum can be recorded from mouse liver in only 1.5min and from human carcinoma liver tissue in less than 3min with effective sample volume of 0.2ml at 14.1T. |
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Authors:
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Xi-An Mao; Ning Li; Jiezhen Mao; Qiurong Li; Nan Xiao; Bin Jiang; Ling Jiang; Xu-Xia Wang; Maili Liu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-8 |
Journal Detail:
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Title: Journal of magnetic resonance (San Diego, Calif. : 1997) Volume: - ISSN: 1096-0856 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9707935 Medline TA: J Magn Reson Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan, Hubei 430071, China; Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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