| Fast detection of Candida albicans and/or bacteria in blood plasma by "sample-self-focusing" using capillary electrophoresis-laser-induced fluorescence. | |
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MedLine Citation:
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PMID: 20363575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Detection of microbial contamination in blood plasma is critical and necessary in different medical and research fields. Most of the current standard procedures for the detection of bacteria and fungi can be time-consuming, for example, direct inoculation methods of microbial cultures in respective growth media can take a few days to several weeks. A fast analysis method with high sensitivity output such as CE-laser-induced fluorescence becomes an attractive alternative. Previously, a spacer-injection method with the use of zwitterionic surfactant (SB3-10) as a blocking agent to negate the cells' mobility, induce aggregation and single microbial peak formation in a buffer solution was reported. Here, a fast, simple direct method for microbial detection in blood plasma without using the spacer and blocking agent is reported. To compensate for the natural electrophoretic heterogeneity of microbes, a CTAB additive was used to sweep all microbial cells towards the plasma peak where a single sharp microbial peak is formed and detected. With the use of BacLight Green bacterial stain, the microbial peak, generally, can be detected within 10 min in front of the plasma peak using capillary electrophoresis coupled with laser-induced fluorescence detection. The LOD of microbes detectable were 5 cells per injection. This technique provides a great advantage over traditional, time-consuming microbial inoculation methods. |
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Authors:
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Man-Yung Tong; Chunxia Jiang; Daniel W Armstrong |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-16 |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 53 ISSN: 1873-264X ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-04-27 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 75-80 Citation Subset: IM |
Copyright Information:
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Copyright 2010. Published by Elsevier B.V. |
Affiliation:
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Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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chemistry* Candida albicans / chemistry* Cetrimonium Compounds / chemistry Electrophoresis, Capillary / methods* Fluorescence* Lasers* Limit of Detection Plasma / chemistry, microbiology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Cetrimonium Compounds; 6899-10-1/cetrimonium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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