| Fast-atom bombardment and thermospray mass spectrometry for the characterization of two glucuronide metabolites of methapyrilene. | |
| | |
MedLine Citation:
|
PMID: 2520227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two conjugated metabolites of methapyrilene hydrochloride isolated from mouse-hepatocytes were examined by mass spectrometry using fast-atom bombardment (FAB) and thermospray ionization. The major metabolite, methapyrilene glucuronide, was identified based on a prominent peak due to the protonated molecule as well as the loss of the dimethylamine and sugar moieties. Identification of the second metabolite was complicated by large signals associated with the biological sample matrix. The complementary nature of the fragmentation observed in the mass spectra using FAB and thermospray ionization allowed this metabolite to be identified as the desmethylmethapyrilene glucuronide. The fragmentation observed using FAB ionization was not greatly affected by the presence of the glucuronide moiety. While loss of the sugar moiety indicated a glucuronide, additional fragmentation confirmed the presence of the underlying ethylenediamine substructure which is characteristic of this class of antihistamines. |
| | |
Authors:
|
J O Lay; T A Getek; D W Kelly; W Slikker; W A Korfmacher |
Related Documents
:
|
2490567 - Assay of antipyrine and its main metabolites 3-hydroxymethylantipyrine, norantipyrine a... 9108647 - An improved method for the measurement of lidocaine and its metabolites in rat plasma. 8667077 - Bifunctional nhs-bat ester for antibody conjugation and stable technetium-99m labeling:... 3771727 - Analysis of epinine and its metabolites in man after oral administration of its pro-dru... 19662187 - Solid phase extraction for monitoring of occupational exposure to cr (iii). 21450537 - The achievement of mass balance by simultaneous quantification of floxuridine prodrug, ... |
Publication Detail:
|
Type: In Vitro; Journal Article |
Journal Detail:
|
Title: Rapid communications in mass spectrometry : RCM Volume: 3 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 1989 Mar |
Date Detail:
|
Created Date: 1992-04-24 Completed Date: 1992-04-24 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 72-5 Citation Subset: IM |
Affiliation:
|
Food and Drug Administration, National Center for Toxicological Research, Jefferson, Arkansas 72079. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Glucuronates / analysis* Liver / metabolism* Mass Spectrometry / methods Methapyrilene / analogs & derivatives*, analysis* Mice Spectrometry, Mass, Fast Atom Bombardment / methods |
| Chemical | |
Reg. No./Substance:
|
0/Glucuronates; 91-80-5/Methapyrilene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Matrix-assisted laser-desorption mass spectrometry using 355 nm radiation.
Next Document: Capillary-zone electrophoresis/fast-atom bombardment mass spectrometry: design of an on-line coaxial...