Document Detail


Fascioliasis transmission by Lymnaea neotropica confirmed by nuclear rDNA and mtDNA sequencing in Argentina.
MedLine Citation:
PMID:  19729246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fascioliasis is widespread in livestock in Argentina. Among activities included in a long-term initiative to ascertain which are the fascioliasis areas of most concern, studies were performed in a recreational farm, including liver fluke infection in different domestic animal species, classification of the lymnaeid vector and verification of natural transmission of fascioliasis by identification of the intramolluscan trematode larval stages found in naturally infected snails. The high prevalences in the domestic animals appeared related to only one lymnaeid species present. Lymnaeid and trematode classification was verified by means of nuclear ribosomal DNA and mitochondrial DNA marker sequencing. Complete sequences of 18S rRNA gene and rDNA ITS-2 and ITS-1, and a fragment of the mtDNA cox1 gene demonstrate that the Argentinian lymnaeid belongs to the species Lymnaea neotropica. Redial larval stages found in a L. neotropica specimen were ascribed to Fasciola hepatica after analysis of the complete ITS-1 sequence. The finding of L. neotropica is the first of this lymnaeid species not only in Argentina but also in Southern Cone countries. The total absence of nucleotide differences between the sequences of specimens from Argentina and the specimens from the Peruvian type locality at the levels of rDNA 18S, ITS-2 and ITS-1, and the only one mutation at the mtDNA cox1 gene suggest a very recent spread. The ecological characteristics of this lymnaeid, living in small, superficial water collections frequented by livestock, suggest that it may be carried from one place to another by remaining in dried mud stuck to the feet of transported animals. The presence of L. neotropica adds pronounced complexity to the transmission and epidemiology of fascioliasis in Argentina, due to the great difficulties in distinguishing, by traditional malacological methods, between the three similar lymnaeid species of the controversial Galba/Fossaria group present in this country: L. viatrix, Galba truncatula and L. neotropica. It also poses a problem with regard to the use, for lymnaeid vector species discrimination, of several molecular techniques which do not show sufficient accuracy, as those relying on the 18S rRNA gene or parts of it, because both L. neotropica and L. viatrix present identical 18S sequence.
Authors:
Roberto Mera y Sierra; Patricio Artigas; Pablo Cuervo; Erika Deis; Laura Sidoti; Santiago Mas-Coma; Maria Dolores Bargues
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-08
Journal Detail:
Title:  Veterinary parasitology     Volume:  166     ISSN:  1873-2550     ISO Abbreviation:  Vet. Parasitol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-10-30     Completed Date:  2010-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7602745     Medline TA:  Vet Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  73-9     Citation Subset:  IM    
Affiliation:
Area de Infectolog??a, Facultad de Ciencias M??dicas, Universidad Nacional de Cuyo, Av. Libertador 80, Mendoza, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
Argentina
Cattle
DNA, Mitochondrial / analysis
DNA, Ribosomal / analysis
DNA, Ribosomal Spacer / genetics
Electron Transport Complex IV / genetics
Fasciola / genetics,  physiology*
Fascioliasis / transmission,  veterinary*
Larva
Lymnaea / anatomy & histology,  genetics,  parasitology*
RNA, Ribosomal, 18S / genetics
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial; 0/DNA, Ribosomal; 0/DNA, Ribosomal Spacer; 0/RNA, Ribosomal, 18S; EC 1.9.3.1/Electron Transport Complex IV

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