Document Detail

Fas and Fas ligand expression in fetal and adult human testis with normal or deranged spermatogenesis.
MedLine Citation:
PMID:  10946867     Owner:  NLM     Status:  MEDLINE    
In mice, the Fas/Fas ligand (FasL) system has been shown to be involved in germ cell apoptosis. In the present study we evaluated the expression of Fas and Fas ligand (FasL) in fetal and adult human testis. Semiquantitative RT-PCR demonstrated the expression of Fas and FasL messenger ribonucleic acids in adult testis, but not in fetal testis (20-22 weeks gestation). In situ RT-PCR and immunohistochemistry experiments on adult human testis demonstrated the expression of FasL messenger ribonucleic acid and protein in Sertoli and Leydig cells, whereas the expression of Fas was confined to the Leydig cells and sporadic degenerating spermatocytes. The number of Fas-positive germ cells per 100 Sertoli cell nuclei was increased in 10 biopsies with postmeiotic germ cell arrest compared to 10 normal testis biopsies (mean, 3.82 +/- 0.45 vs. 2.02 +/- 0.29; P = 0.0001), but not in 10 biopsies with meiotic germ cell arrest (mean, 1.56 +/- 1.07). Fas and FasL proteins were not expressed in cases of idiopathic hypogonadotropic hypogonadism. Together, these findings may suggest that Fas/FasL expression in the human testis is developmentally regulated and under gonadotropin control. The increased germ cell expression of Fas in patients with postmeiotic germ cell arrest suggests that the Fas/FasL system may be involved in the quality control mechanism of the produced gametes.
S Francavilla; P D'Abrizio; N Rucci; G Silvano; G Properzi; E Straface; G Cordeschi; S Necozione; L Gnessi; M Arizzi; S Ulisse
Related Documents :
21293487 - Sorafenib induces cell death in chronic lymphocytic leukemia by translational downregul...
2465117 - Analysis of germ line development in the chick embryo using an anti-mouse ec cell antib...
7623867 - Germ line specific factors in chemical mutagenesis.
15350607 - Lactate and energy metabolism in male germ cells.
25400647 - Danger signals - damaged-self recognition across the tree of life.
21130147 - In vitro toxicity evaluation of graphene oxide on a549 cells.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  85     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-08-31     Completed Date:  2000-08-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2692-700     Citation Subset:  AIM; IM    
Department of Internal Medicine, University of L'Aquila, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Abortion, Therapeutic
Antigens, CD95 / genetics*
Fas Ligand Protein
Gestational Age
Leydig Cells / immunology
Membrane Glycoproteins / genetics*
Middle Aged
Oligospermia / genetics,  physiopathology*
Reverse Transcriptase Polymerase Chain Reaction
Sertoli Cells / immunology
Testis / embryology,  physiology*,  physiopathology
Transcription, Genetic
Reg. No./Substance:
0/Antigens, CD95; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Fasl protein, mouse; 0/Membrane Glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Twenty-four-hour leptin levels respond to cumulative short-term energy imbalance and predict subsequ...
Next Document:  Molecular analysis of LHX3 and PROP-1 in pituitary hormone deficiency patients with posterior pituit...