Document Detail


Fas activation in adipocytes impairs insulin-stimulated glucose uptake by reducing Akt.
MedLine Citation:
PMID:  20828573     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fas (CD95) belongs to the superfamily of the tumor necrosis factor (TNF) receptors. Besides its key role in apoptosis, Fas contributes to non-apoptotic pathways such as cell proliferation and inflammation. In 3T3-L1 adipocytes, activation of Fas by Fas ligand decreased insulin-stimulated glucose uptake, without affecting cell viability. This decrease in glucose uptake was accompanied by reduced protein expression and diminished phosphorylation of Akt. Similarly, insulin-stimulated glucose incorporation and protein levels of Akt were increased in isolated adipocytes from Fas deficient mice when compared to wild-type mice. In conclusion, Fas activation in adipocytes decreases Akt expression and thereby impairs insulin sensitivity.
Authors:
Stephan Wueest; Reto A Rapold; Eugen J Schoenle; Daniel Konrad
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-07
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2010-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  4187-92     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Affiliation:
Division of Pediatric Endocrinology and Diabetology, University Children's Hospital, Zurich, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
3T3-L1 Cells
Adipocytes, White / drug effects*,  metabolism*
Animals
Antigens, CD95 / deficiency,  genetics,  metabolism*
Biological Transport, Active / drug effects
Fas Ligand Protein / metabolism,  pharmacology
Glucose / metabolism*
Insulin / pharmacology*
Mice
Mice, Inbred C57BL
Mice, Knockout
Proto-Oncogene Proteins c-akt / metabolism*
Signal Transduction / drug effects
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/Fas Ligand Protein; 0/Fas protein, mouse; 0/Fasl protein, mouse; 11061-68-0/Insulin; 50-99-7/Glucose; EC 2.7.11.1/Proto-Oncogene Proteins c-akt

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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